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乙型肝炎病毒基因生物信息学分析及特异片段的制备 被引量：2

Bioinformatic Analysis of HBV Gene and Preparation of Specific Fragment
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摘要 目的制备HBV基因分型芯片模板。方法通过不同基因型HBV全基因组的序列比对,选取6个碱基位点作为分型位点,应用重叠区扩增基因拼接法(SOEing法)对C型HBV片段进行点突变。结果在所选位点上成功引入点突变。结论SOEing法是一种简便引入点突变的方法,可以应用此法制备HBV基因分型检测基因所需的模板DNA。 Objective To prepare HBV DNA fragments for HBV genotypes used as templates. Methods Six bases were chosen for genotyping by comparing different HBV genome sequences. The fragments of HBV genotype C were mutated by using gene splicing by overlap extension （gene SOEing）. Results The chosen sites were successfully mutated. Conclusion Gene SOEing is a convenient way to introduce mutation, and this method can be used to prepare HBV specific sequences that can be used as templates.
作者 徐晓雪 房凯 钟连声 潘忠诚 李超 胥威 赵雨杰
机构地区 中国医科大学基础医学院生物芯片中心
出处 《中国医科大学学报》 CAS CSCD 北大核心 2008年第5期583-585,共3页 Journal of China Medical University
基金 辽宁省教育厅高校科研基金资助项目(202011001)
关键词 重叠区扩增基因拼接法 乙型肝炎病毒 基因分型 gene splicing by overlap extension HBV genotyping
分类号 R512.6 [医药卫生—内科学]
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参考文献8

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二级参考文献4

共引文献11

同被引文献18

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中国医科大学学报
2008年 第5期

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