cofilin在子宫内膜异位症患者在位内膜及异位病灶中的表达及意义

Expression and Significance of Cofilin in Eutopic Endometrium and Ectopic Foci in Patients with Endometriosis

目的检测在位内膜中子宫内膜异位症的候选基因及功能,通过对在位子宫内膜中肌动蛋白结合因子(cofilin)表达的检测,明确其在子宫内膜异位症发生和发展中的作用。方法选取23例经病理证实为子宫内膜异位症的在位内膜及异位内膜标本(增殖期11例,分泌期12例)为实验组,以9例非子宫内膜异位症患者的子宫内膜标本为对照组。采用RT-PCR方法检测cofilin mRNA的相对表达。并对子宫内膜异位症患者在位内膜中cofilin表达水平与内异症严重程度及月经周期进行相关性分析。结果实验组在位内膜中cofilin表达水平明显高于对照组在位内膜(P<0.01);实验组异位内膜cofilin表达水平高于同组在位内膜(P<0.05);实验组在位内膜及异位内膜相比较,中、重度组与轻度组的差别有显著性(P<0.05)。实验组在位内膜及异位内膜和对照组在位内膜的增殖期和分泌期比较,cofilin表达水平均无显著性差异(P>0.05);分别比较实验组与对照组增殖期及分泌期在位内膜差异具有统计学意义(P<0.05);实验组中,分别比较增殖期与分泌期在位内膜与异位内膜,cofilin表达水平也具有统计学差异(P<0.05)。结论子宫内膜异位症患者在位内膜中cofilin表达水平的异常升高在该病的发生发展中起重要作用。

Objective To explore the candidate gene and its function in the eutopic endometrium of endometriosis through detecting the expression of codlin in endometrium and identifying the role of cofilin in the occurrence and development of endometriosis. Methods The eutopie and ectopie endometria sampled from 23 patients with endometriosis were chosen as experimental group, and the eutopic endometria from 9 patients without endometriosis was taken as control group. The expression of cofilin mRNA was detected by RT-PCR,and the correlation between cofilin expression in eutopie endometria and the severity of endometriosis was analyzed. The codlin expressions in the proliferativeand secretory phases were compared in experimental group. Results The expression of cofilin in eutopie endometria in experimental group was significantly higher than that in control group （P 〈 0.01）. In experimental group,the expression of cofilin was higher in ectopic endometria than in eutopic endometria （P 〈 0.05 ）, and there was significant difference in the expression of eotilin in eutopic and ectopic endometria between patients with moderate to severe endometriosis and those with mild endometriosis （P 〈 0.05 ）. There was no significant difference in the expression of cofilin in eutopic and ectopic endometria in experimental group and in eutopie endometria in control group between proliferative and secretory phases （P 〉 0.05 ）. There was significant difference in the expression of cofilin in eutopic endometria during proliferative and secretory phases between experimental and control groups （P 〈 0.05 ）. The difference in the expression of cofilin in eutopie and ectopic endometria was also significant between proliferative and secretory phases in experimental group （P 〈 0.05）. Conclusion The unusually high level of cofilin expression in the eutopic endometria in patients with endometriosis may play an important role in the occurrence and development of endometriosis.