酶法提取竹荪深层发酵菌丝体多糖的研究

Extraction of the Submerged Myceliun Polysaccharideof Dictyophora indusiata by Complex Enzymatic Technique

本文讨论了竹荪深层发酵菌丝体多糖的提取方法，考察了多糖及还原性寡糖的含量。实验结果表明，利用夏合酶法处理结合热水浸提的提取方法，能显著提高可溶于热水的多糖及还原性寡糖的浸提率，且缩短浸提时间，竹荪多糖提取率达４．５８％（ｌｇ多糖／１００ｇ菌丝干粉），总糖提取率达到ｌ．８４％ｃ。同时还考察了酶法提取的最佳条件。

Several methods for extraction of the submerged mycelium polysaccharide of Dictyophora indusiataare discussed in this paper.The experiment results show that the yield of polysacchrides from 1 gram of dri mycelium with enzymatic treatment id 0.0458 gram;the total sugar content is 0.1184 gram.Compared with other extraction methods,enzymatic treatment made yield of polysaccharide increasing and extraction time shorten.In this paper,the optimum of enzymatic treatment is also studied.