5-羟色胺转运体基因多态性和抑郁症的发病、性别、严重程度及自杀行为的相关性

Correlation of serotonin transporter gene polymorphism with episode,gender,severity and suicidal behavior of depression

目的探讨中国汉族人群5-羟色胺转运体启动区(5-HTTLPR)基因多态性和抑郁症的发病、性别、严重程度及自杀是否相关。方法应用聚合酶链式反应(PCR)扩增技术测定150例抑郁症患者和150例正常对照者的5-HT-TLPR基因型和等位基因,分别验证各种基因型与中、重度抑郁症发病、性别及自杀行为的相关性。结果病例组SS、LS基因型及S等位基因频率均高于对照组(26.0% vs.20.0%;52.7% vs.46.0%;52.3% vs.43.0%;P均<0.05);两组性别分层比较,女性S等位基因频率高于对照组(55.3% vs.43.6%,P<0.05);病例组严重程度分层比较差异无统计学意义(P>0.05);病例组有无自杀行为分层比较,有自杀行为患者SS基因型频率(37.3% vs.20.2%)及S等位基因频率(61.8% vs.47.5%)均高于无自杀行为患者(P<0.05);抑郁自杀组性别分层比较未显示显著性差异(P>0.05);抑郁自杀组病情程度分层比较,重度抑郁自杀者SS基因型频率(42.5% vs.18.2%)及S等位基因频率(68.7% vs.36.4%)均高于中度抑郁自杀者(P<0.05)。结论在中国汉族人群中,5-HTTLPR多态性和抑郁症相关。S等位基因可能是抑郁症的易感基因,特别是女性,SS型可能是抑郁症易感基因型;S等位基因可能是抑郁症患者自杀的危险基因,SS基因型人群可能是抑郁症患者自杀的危险人群,特别是携带S等位基因的重度抑郁症患者更易自杀。

Objective To explore whether there is any association of polymorphism of serotonin transporter gene-linked promoter region with episode, gender, severity and suicidal behavior of depression in Chinese Han people. Methods Polymerase chain reaction （PCR） was used to detect the distribution frequency of serotonin transport gene-linked promoter region polymorphisms of 150 depressive patients （patient group） and 150 normal people （control group）. Results There were significant differences between the patients with depression and the controls （P〈0.05）. The frequency of SS, LS genotypes （26.0% vs. 20.0%; 52.7% vs. 46.0%） and S allele （52.3% vs. 43.0%） in patients was higher than that in the control group. There was a significant difference in 5-HTTLPR alleles between the depressive women and the control group （P〈0.05）, the frequency of depressive women with S allele （55.3% vs. 43.6%） was higher than that in the controls. Significant differences could be found between the depressive patients with and without suicidal behavior （P〈0.05）, but not depression of different severity （P〈0.05）. The frequency of SS genotype （37.3% vs. 20.2%） and S allele （61.8% vs. 47.5%） in suicidal patients was higher than that in non-suicidal ones. There was no significant difference between the different gender depressive patients with suicidal behavior （P〉0.05）, but there was significant difference between different-severity depression with suicidal behavior （P〈0.05）. The frequency of SS genotype （42.5% vs. 18.2%） and S allele （68.7% vs. 36.4%） in major depressive patients with suicidal behavior was higher than that in moderate ones Conclusion The polymorphism of 5-HTTTLPR is associated with depression in Chinese Han people. S allele may be depressive predisposing gene, especially in women. SS genotype may be associated with episode of depression Patients of S allele and SS genotype, especially major depressive patients with S allele, may have suicidal behavior.