摘要
目的探讨重组人脑利钠肽(rhBNP)对心肌梗死后大鼠心室重构及心功能的影响。方法建立急性心肌梗死(AMI)雄性SD大鼠模型45只,随机分为AMI对照组、小剂量rhBNP治疗组、大剂量rhBNP治疗组,每组各15只。另设15只作假手术组。rhBNP治疗组经颈静脉输液管输注rhBNP,剂量分别为5μg/kg和15μg/kg,1次/d,持续4周,假手术组及AMI对照组仅以等体积的生理盐水输注。4周后检测血液动力学及心功能参数,心脏标本检测左室重量及左室重量指数(LVMI)、心肌梗死面积,并检测血浆和心肌血管紧张素Ⅱ(AngⅡ)水平。结果AMI对照组左室重量、LVMI及心肌AngⅡ水平明显高于假手术组,而收缩压及左室内压最大上升和下降速率(±dp/dt)均明显低于假手术组(均P〈0.01)。大、小剂量rhBNP治疗组左室重量及LVMI、心肌梗死面积和心肌AngⅡ水平均明显低于AMI对照组[左室重量:分别为(492.6±34.0)mg、(498.8±47.8)mg比(570.0±24.2)mg,P〈0.01;LVMI:2.0±0.2、2.0±0.2比2.3±0.1,P〈0.01;心肌梗死面积:(25.3±2.9)%、(31.4±3.0)%比(46.4±3.0)%,P〈0.01;AngⅡ水平:(881.3±62.7)pg/L、(1186.0±94.5)pg/L比(2436.7±280.3)pg/L,P〈0.05],收缩压和±dp/dt也高于AMI对照组(P〈0.01或P〈0.05)。结论外源性的rhBNP连续应用能限制梗死面积、减轻AMI后心室重构、保护心功能。
Objective To assess the effects of rhBNP on left ventricular (LV) remodeling in rats with acute myocardial infarction ( AMI ). Methods AMI was induced by ligating coronary arteryin male Sprague Dawley rats. Two days after surgery, AMI rats received intravenous infusion of rhBNP ( 15 μg/kg or 5 μg/kg once daily, n = 15 each) or saline (placebo control, n = 15 ) through Jugular Vein. Sham-operated rats (n = 15 ) served as normal control. Four weeks later, hemodynamic measurements were performed, left ventricular weight ( LVW), ratio of left ventricular weight to body weight ( LVW/BW), left ventricular diameter (LVD) and infarct size were determined. Plasma angiotensin Ⅱ and myocardial angiotensin Ⅱ levels were also measured. Results Compared with sham-operated rats, LVW, LVW/BW, LVD and myocardial angiotensin Ⅱ level were significantly increased, while the LV systolic pressure (LVSP) , ± dp/dt were significantly reduced in saline treated AMI rats ( all P 〈 0. 05 ) . LVW/BW, MI size, LVD and myocardial angiotensin Ⅱ in rhBNP treated AMI rats were significantly lower [ LVW: (492. 6 ± 34. 0)mg, (498.8±47.8)mg, (570.0±24.2)mg,P〈0.01; LVW/BW: 2.0 ±0.2, 2.0±0.2, 2.3 ±0.1,P〈 0. 01 ; LVD : ( 25.3 ± 2. 9 ) %, ( 31.4 ± 3.0 ) %, ( 46.4 ± 3.0 ) %, P 〈 0. 01 ; myocardial angiotensin Ⅱ : (881.3 ±62. 7) pg/L, (1186. 0 ±94. 5) pg/L, (2436. 7 ±280. 3) pg/L, P〈0. 05], while LVSP and ± dp/dt in rhBNP treatment groups were significantly increased than saline treated AMI rats ( P 〈 0. 05 or P 〈 0. 01 ). Conclusion RhBNP is effective in attenuating left ventricular remodeling after AMI in rats.
出处
《中华心血管病杂志》
CAS
CSCD
北大核心
2008年第12期1097-1100,共4页
Chinese Journal of Cardiology
基金
广东省科技计划资助项目(2008B030301088)
关键词
心肌梗死
利钠肽
脑
心室重构
Myocardial infarction
Natriuretic peptide, brain
Ventricular remodeling