大豆苯丙氨酸解氨酶及其基因的研究进展

Advances of Studies on Soybean Phenylalanine Ammonia-lyase and its Gene

苯丙氨酸解氨酶(phenylalanine ammonia-lyase,PAL)是连接植物初级代谢和苯丙烷类代谢等次级代谢途径关键酶、限速酶。综述了大豆PAL及其基因的研究进展,主要包括大豆PAL的分布及活性、分离纯化及酶活性的测定、对植物生理代谢的意义、在抗病中的作用机制、基因克隆、表达特性、基因的应用展望等。

Phenylalanine ammonia-lyase（PAL） was the key enzyme and rate-limitting enzyme that linked primary metabolism to phenylpropanoid metabolism. The review summerized the research advances of studies on soybean phenylalanine ammonia- lyase and its gene, including the distribution and enzyme aetivity, method of purification and enzyme assay, function in physiological metabolism and disease-resistance ;gene expression eharaeteristies, gene cloning and application prospect.