NaCl处理对野大豆生理生化特性的影响

Effects of NaCl Stress on Physiological and Biochemical Characters of Glycine soja

为明确NaCl处理条件下野大豆叶片抗氧化酶和有机渗透调节物质的变化,以不同浓度NaCl处理野大豆幼苗,对其抗氧化酶活性和渗透调节物质含量的变化进行分析。结果显示:随着NaCl浓度的升高,野大豆叶片中超氧化物歧化酶(SOD)、过氧化物酶(POD)活性先升高后降低,而过氧化氢酶(CAT)的活性呈持续下降趋势;丙二醛(MDA)含量在低盐度处理范围内变化不明显,高浓度NaC l处理时明显高于对照;随着NaCl浓度的升高,渗透调节物质脯氨酸含量增加,可溶性糖(SS)呈现先降低后升高的趋势。研究表明:在NaC l处理下野大豆主要通过SOD、POD清除活性氧,脯氨酸可能起到主要的渗透调节作用。

The antioxidative system and osmotic regulation are two main factors affecting plant progress under NaCl stress. The effects of NaCl stress on the antioxidative system and osmotic regulation in the seedlings of Glycine soja were studied in this paper. The results showed that the activities of superoxide dismutase（SOD） ,peroxidase（POD） had similar trend,which was increasing firstly,then decreasing with NaCl concentrations from 0 up to 200 mmol·L^-1. The catalase（CAT） activity decreased with the increasing of NaCl concentrations;The contents of MDA not varied evidently firstly and then increased evidently（ 100-200 mmol· L-1 NaCl）. The contents of MDA were the highest when the NaCl concentration was 200 mmol· L-1. The contents of proline increased with the increasing of NaCl concentrations, and soluble sugar decreased firstly, then increased evidently with the increasing of NaCl concentrations as well. It was thus suggested that POD and SOD and osmotic regulation capacity of proline（Pro） play an important role in Glycine soja resistant to NaCl stress.