整合素αV和β3反义基因抑制大鼠胰腺癌生长的实验研究

Antisense intergrin αV and integrin β3 suppresses the growth of pancreatic carcinoma in rats

目的:探讨整合素αV和β3反义基因对大鼠胰腺癌生长及肿瘤血管生成和细胞凋亡的影响。方法:建立大鼠胰腺癌模型,应用电转染方法分别用整合素αV、β3及αVβ3反义基因治疗,观察治疗后抑瘤率并应用免疫组织化学技术检测肿瘤微血管密度变化,TUNEL法检测肿瘤细胞凋亡情况。结果:对照组、αV组、β3组及αVβ3组肿瘤质量分别为(1.17±0.79)g、(0.95±0.26)g、(1.013±0.42)g和(0.79±0.56)g,各组间差异有统计学意义(P<0.05);微血管密度分别为(18.33±1.39)、(13.80±1.06)、(15.96±1.24)和(12.16±1.23),各组间差异有统计学意义(P<0.05);肿瘤细胞凋亡指数分别为(12.30±1.393)、(22.17±1.23)、(20.37±1.07)和(24.10±0.99),各组间差异有统计学意义(P<0.01)。结论:整合素αV、β3反义基因对大鼠胰腺癌的血管生长具有明显的抑制作用,可促进肿瘤细胞的凋亡,进而影响肿瘤生长,联合应用整合素αVβ3对肿瘤的生长抑制作用更为显著。

Objective： To investigate antisenSe integrin αV and β3 gene therapy in Rats model of Pancreatic Carcinoma. Methodsz The antisense integrin α V and β3 expression plasmids were electrobloted into pancreatic tumours of rats induced by dimethylbenxanthracine,The tumor inhibit rate was calculated,the tumor microvessel density（MVD）with the immunohistochemical staining and tumor apoptosis with TUNEL were examined.Redults：The tumor weight of the control group αV group,β3group and αVβ3 group was （1.167±0.79）g,（0.953±0.26）g,（1.013±0.42）g,（0.788±0.56）g respectively.The difference was significant among them（P〈0.05）.The MVD was （18.33±1.39）,（13.80±1.06）,（15.96±1.24）,（12,16±1.23）respectively,there was significant difference among them（P〈0.05）.The Al was （12.30±1.393）、（22.17±1.23）,（20.37±1.07）,（24.10±0.99）respectively and there was significant difference among them（P〈0.05）.Conclusions：Antisense gene therapy targeting integrin αV and β3 should be considered as an approach to suppress the growth of pancreatic carcinoma.