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实时荧光定量PCR检测孕妇血浆中胎儿RhCcEe基因 被引量：2

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摘要 Rh血型系统在临床输血中的重要性仅次于ABO血型系统，Rh血型不合可引起溶血性输血反应及新生儿溶血病（HDN）。Lo等研究证实孕妇血浆或血清中含有胎儿DNA，国外已用游离胎儿DNA开展了非创伤性产前诊断Rh基因的研究。我们曾用PCR扩增孕妇血浆中胎儿DNA，以检测胎儿RhCcEe基因。此外，因实时荧光定量（FQ）-PCR比PCR技术的灵敏度高，我们尝试用FQ—PCR检测孕妇血浆游离胎儿DNA的RhCcEe基因型。
作者 何吉 秦斐 朱发明 戴兵 陈舒 刘晋辉 严力行
机构地区 浙江省血液中心卫生部血液安全研究重点实验室
出处 《中华检验医学杂志》 CAS CSCD 北大核心 2008年第12期1365-1366,共2页 Chinese Journal of Laboratory Medicine
基金 浙江省医药卫生科学研究基金资助项目（2007A043）
关键词 荧光定量PCR检测 游离胎儿DNA 孕妇血浆 e基因型 非创伤性产前诊断 ABO血型系统 溶血性输血反应 RH血型系统
分类号 R686 [医药卫生—骨科学]
  • 相关文献

参考文献10

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二级参考文献11

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共引文献7

同被引文献17

  • 1骆媛,孙玉英,奚永志.人类白细胞抗原分子遗传中的基因重组[J].中华医学遗传学杂志,2005,22(4):427-430. 被引量：11
  • 2骆媛,孙玉英,金荔,梁飞,刘楠,宋新强,袁方,刘曙光,刘金锋,奚永志.一种罕见的人类白细胞抗原HLA-B/Cw座位间的基因重组[J].中华医学杂志,2006,86(9):628-631. 被引量：8
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引证文献2

二级引证文献4

中华检验医学杂志
2008年 第12期

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