摘要
目的:探讨免疫层析法与酶联免疫法(ELISA)检测乙型肝炎病毒(HBV)表面抗原(HBsAg)和表面抗体(抗-HBs)符合率。方法:分别用免疫层析法和酶联免疫法检测待检儿童血清HBsAg和抗-HBs,将两法检测结果做以分析、比较。结果:400例标本中,胶体金法和ELISA法检测HBsAg结果差异无统计学意义,两法符合率为97.25%;乳胶层析法和ELISA法检测抗-HBs结果差异有统计学意义,两法符合率为79.25%。结论:免疫层析法简便快速,但存在一定假阳性或假阴性率,不能完全替代ELISA法进行乙肝病毒血清标志物的检测,适合在不具备ELISA法检测条件的基层医疗单位和标本初筛使用。
ObjectiveTo compare the two methods, rapid test stripe （colloidal gold stripe,latex rapid test stripe） and ELISA,for testing HBsAg and anti-HBs. Methods HBsAg and anti-HBs were tested of 400 serum samples with rapid test stripe and ELISA and the results were compared. Results The results showed that the difference of HBsAg positive rate of colloidal gold stripe and ELISA was not statistically significant and the accordance rate of the two methods was 97.25%. The difference of anti--HBs positive rate of latex rapid test stripe and ELISA was statistically significant and the accordance rate of the two methods was 79.25%. Conclusions Rapid test stripe is convenient and quick, hut there were false judgments sometimes, so it can not replace ELISA to test serum HBV markers completely. It canbe used in based medical organizations with no capacity of testing HBV with ELISA and can be used in first screening samples.
出处
《中国农村卫生事业管理》
2008年第12期893-895,共3页
Chinese Rural Health Service Administration
