高效液相色谱法快速测定人参中8种主要皂苷类成分的含量

High performance liquid chromatography in rapid determination of eight ginsenosides in the root of Panax ginseng

目的:采用高效液相色谱法测定人参Panaxginseng C.A.Mey.中8种主要皂苷类成分的含量。方法:人参药材以甲醇超声30min提取。采用Agilent Zorbax SB-C18色谱柱(3.0mm×100mm,3.5μm),流动相为乙腈(A)和水(B),梯度洗脱;A相含量随时间的变化为18%～20%(0～14min),20%～29%(14～20min),29%(20～25min),29%～37%(25～34min),37%～55%(34～40min);流速0.6ml/min;检测波长203nm;柱温25℃;进样量10μl。结果:8种人参皂苷Rb1、Rb2、Rb3、Rc、Rd、Re、Rf、Rg1在35min内基线分离,线性良好(r=0.9999)。方法学考察表明,日内日间精密度,最低检测限和定量限的范围均符合相关标准,加样回收率(n=3)分别为101.07%、98.72%、101.57%、101.71%、102.12%、99.58%、98.62%、100.12%。测定了3个厂家6个批次人参药材中8种人参皂苷的含量。结论:该方法快速简便,稳定可靠,可以用来对人参药材进行质量控制。

Objective：To determine the contents of eight ginsenosides in the root of Panax ginseng by high performance liquid chromatography （HPLC）. Methods： Panax ginseng was extracted with methanol solution for 30 min. The HPLC condition was as follows-column： Agilent SB-C18 （3. 0 mm × 100 mm, 3.5 μm） ; mobile phase： A was ACN, B was H2O, with gradient elution;the gradient of A phase： 18%-20% （0-14 min）, 20%0-29% （14-20 min）, 29% （20-25 min）, 29%-37% （25-34 min） ,37%-55%（34-40 min） ; flow speed：0.6 ml/min; detection：203 nm; temperature of column：25℃ ;injection volume：10 μl. Results：Eight ginsenosides （Rb1 ,Rb2 ,Rb3 ,Rc,Rd,Re,Rf and Rg1） were separated at baseline within 35 min with good linearity （r=0. 9999）. The result of intra-day and inter-day preeisions,limits of detection and quantitation were all within the normal range. The recovery rates（n：3） were 101.07%,98.72%,101.57%,101.71%,102.12%,99.58%,98.62%,and 100.12%, respectively. The contents of eight ginsenosides in 6 different batches of Panax ginseng from 3 sources were determined. Conclusion：The present method is rapid,simple,accurate and can be used to control the quality of Panax ginseng.