摘要
目的:采用高效液相色谱法测定人参Panaxginseng C.A.Mey.中8种主要皂苷类成分的含量。方法:人参药材以甲醇超声30min提取。采用Agilent Zorbax SB-C18色谱柱(3.0mm×100mm,3.5μm),流动相为乙腈(A)和水(B),梯度洗脱;A相含量随时间的变化为18%~20%(0~14min),20%~29%(14~20min),29%(20~25min),29%~37%(25~34min),37%~55%(34~40min);流速0.6ml/min;检测波长203nm;柱温25℃;进样量10μl。结果:8种人参皂苷Rb1、Rb2、Rb3、Rc、Rd、Re、Rf、Rg1在35min内基线分离,线性良好(r=0.9999)。方法学考察表明,日内日间精密度,最低检测限和定量限的范围均符合相关标准,加样回收率(n=3)分别为101.07%、98.72%、101.57%、101.71%、102.12%、99.58%、98.62%、100.12%。测定了3个厂家6个批次人参药材中8种人参皂苷的含量。结论:该方法快速简便,稳定可靠,可以用来对人参药材进行质量控制。
Objective:To determine the contents of eight ginsenosides in the root of Panax ginseng by high performance liquid chromatography (HPLC). Methods: Panax ginseng was extracted with methanol solution for 30 min. The HPLC condition was as follows-column: Agilent SB-C18 (3. 0 mm × 100 mm, 3.5 μm) ; mobile phase: A was ACN, B was H2O, with gradient elution;the gradient of A phase: 18%-20% (0-14 min), 20%0-29% (14-20 min), 29% (20-25 min), 29%-37% (25-34 min) ,37%-55%(34-40 min) ; flow speed:0.6 ml/min; detection:203 nm; temperature of column:25℃ ;injection volume:10 μl. Results:Eight ginsenosides (Rb1 ,Rb2 ,Rb3 ,Rc,Rd,Re,Rf and Rg1) were separated at baseline within 35 min with good linearity (r=0. 9999). The result of intra-day and inter-day preeisions,limits of detection and quantitation were all within the normal range. The recovery rates(n:3) were 101.07%,98.72%,101.57%,101.71%,102.12%,99.58%,98.62%,and 100.12%, respectively. The contents of eight ginsenosides in 6 different batches of Panax ginseng from 3 sources were determined. Conclusion:The present method is rapid,simple,accurate and can be used to control the quality of Panax ginseng.
出处
《第二军医大学学报》
CAS
CSCD
北大核心
2008年第12期1507-1510,共4页
Academic Journal of Second Military Medical University
关键词
人参
人参皂苷
高效液相色谱法
含量测定
Panax ginseng
ginsengosides
high performance liquid chromatography
determination