羊肺炎霉形体病研究

Study on Mycoplasmosis Ovipneumoniae

１９８０～１９９２年，从四川、云南两省６个种羊场的进口种羊和阿坝、凉山、甘孜州共１０个县的本地绵羊和山羊病肺中分离出２５株霉形体。经理化特性鉴定、药敏试验、致病性测定，并与绵羊肺炎霉形体国际参考株Ｍ·Ｏ·Ｙ９８用生长抑制试验定型，证明这２５株霉形体均为绵羊肺炎霉形体（Ｍｙｃｏｐｌａｓｍａｏｖｉｐｎｅｕｍｏｎｉａｅ）。用微量间接血凝试验（ＩＨＡ）检测，Ｍ·Ｏ·Ｙ９８抗原与丝状霉形体山羊亚种Ｃ８７、丝状霉形体丝状亚种Ｃ８８、羊衣原体、布氏杆菌和羊副结核的抗体均无交叉反应。ＩＨＡ对人工感染羊和有呼吸道症状的自然病例的阳性检出率分别为９５％和８２．６％。试验表明，ＩＨＡ是一种简易、快速而可靠的诊断方法，适用于本病的群体检疫。对人工感染和自然感染发病羔羊进行药物防治试验，证明枝原净效果最好，剂量为每日每公斤体重２２．５ｍｇ，可以抑制肺炎病变的发展。２个试验组的肺炎实变平均值为０．１５±０．２８和０．５８±０．６３，而２个对照组则高达１１．９０±１４．０４和１４．５０±１２．６４。

During 19801992, strains of Mycoplasma isolated from pneumonia lungs of imported sheep come from six breeding farms in Sichuan and Yunnan, and local sheep and goat come from Aba, Liangshan and Ganzi areas, were proved to be M.ovipneumoniae by detection of the chemical and physical characteristics, drug susceptibility tests, and the virulence and species identification by means of growth inhibition tests with strain of M. ovip. Y98. There were no cross reactions between the M. ovip. Y98 antigen and antisera to M. mycoides subsp. capri C87, M. mycoides subsp. mycoides C88; Chlamydiales. ovi, Brucella and Mycobacterium oviparatuberculosis by IHA. The IHA posttive rates were 95% and 82.6% in flocks of experimentally or naturally infected with M. ovipnemoniae indicating that the IHA test to be a feasible, rapid and truthful assay for diagnosis of mycoplasmosis ovipnenmoniae. Tlamutin was proved to be the most effective drug against M. ovip. which could inhibit the development of pneumonia lesions at a dose of 22.5 mg/kg every day, with the means of pneumonia score being 0.15±0.28 and 0.58±0.63 versus 11.90±14.04 and 14.50±12.64 in experimental and control groups.