门脉高压患者血浆刺激血管内皮细胞Hsp90表达的试验研究

Plasma from patients with portal hypertension stimulates heat shock protein90 in human vascular endothelial cell in vitro

目的:研究肝硬化门静脉高压症患者血浆对血管内皮细胞热休克蛋白(Hsp90)表达的影响,以了解Hsp90在肝硬化门脉高压形成中的作用。方法:应用免疫组织化学染色法检测Hsp90在正常人血浆和肝硬化门静脉高压症患者血浆刺激人脐静脉血管内皮细胞(HUVEC)中的表达情况。结果:通过组织化学染色图像定量分析显示肝硬化患者血浆处理的HUVEC表达的Hsp90平均灰度值为1.925,正常人血浆处理为1.416,经统计学分析肝硬化患者血浆处理HUVEC表达的Hsp90高于正常人血浆处理组(P<0.05)。结论:肝硬化门脉高压血浆可以刺激血管内皮细胞使Hsp90表达增加,Hsp90可能促进肝硬化门脉高压的形成。

Objective ：To study plasma from patients with portal hypertension stimulates human umbilical vascular endothelialcell （HUVEC） endothelial Hsp90 expression and understand it role. Methods ： Immunohistochemistry stain was performed to detect the expression of Hsp90 protein after HUVEC were stimulated by plasma from patients with portal hypertension and from normal subjects ,respectively. Results：Stained image through quantitative analysis showed that patients with cirrhosis of the HUVEC deal with the expression of Hsp90,the average value of gray 1. 925,1. 416 for normal plasma processing,the statistical analysis of patients with cirrhosis with the expression of Hsp90 than HUVEC treatment group was higher than normal plasma normal plasma-treated group （P〈0. 05）. Conclusion：Cirrhosis of portal hypertension in patients with vascular endothelial cells can be stimulated to Hsp90 expression. Hsp90 may promote the formation of liver cirrhosis and portal hypertension.