摘要
目的:研究Survivin基因在急性白血病(AL)患者中的表达及临床意义。方法:应用RT-PCR检测64例AL患者Survivin mRNA的表达水平,14例健康人为正常对照,K562、HL-60细胞株为阳性对照。结果:Survivin的阳性表达率高于正常对照,且在初治AL中的表达水平较正常对照组明显升高,治疗缓解后Survivin表达下降,复发时表达水平升高在初治AL患者中Survivin阳性患者缓解率低于表达阴性的患者。结论:Survivin基因表达的紊乱可能在AL的发病中起着重要作用,并且与AL复发和预后相关。
Objective：To investigate the expression of Survivin mRNA in acute leukemia and the prognostic significance in AL. Methods ：The expression of survivin mRNA was measured in 64 AL patients with semi-quantity reverse transcription RT-PCR. Fou adults were tested as normal eontrol（NC） and K562,NB4 cell lines were also tested as positive control. Results ：The positive rates of Survivin was higher than NC. The expression of Survivin in de novo AL was higher than that in NC,while it decreased in complete remission（CR） patients. In relapsed patients the expression of survivin increased again. In AL patients ,the CR rate of Survivin positive cases was lower than that of Survivin negative cases. Conclusion ：Abnormal expression of Survivin genes was related to pathogenesis and progression of AL and it can serve as a marker of relapse and poor prognosis in AL.
出处
《临床医药实践》
2008年第12期968-969,共2页
Proceeding of Clinical Medicine
