摘要
本研究将最新的病毒核酸纯化技术和单管RT-PCR技术相结合,应用于IBDV的诊断研究,对40余个病毒样品进行扩增反应,均取得令人满意的效果。该技术灵敏快速,从核酸纯化到电脉检测PCR产物只需5~6h,反应灵敏度比传统方法至少提高100倍。所设计的引物对毒株的适用范围较广。
A novel reverse transcription polymerase chain reaction (RTPCR)amplification assay was developed to detect infectious bursal disease virus (IBDV) gene sequences in bursal samples and cell cultures.One pair of primer were designed to amplify a fragment of segment A gene that partialy code for the IBDV protiens VP4.Analysis of 12 strains of IBDV and 12 bursal samples all yielded the specific 365bp fragments.The assay was a combination of a new developed virus RNA purification technique and TitanTm one tube RTPCR system,that could gain detecting result within 5 ̄6 hours.It was 100 times more sensitive than the tranditional two step two tube RTPCR assay,and was easy to perform thus is suitable for the treatment of large samples in animal quarantine.
出处
《中国兽医杂志》
CAS
北大核心
1998年第1期3-7,共5页
Chinese Journal of Veterinary Medicine