趋化因子受体CCR3、CCR5和CXCR3与自然流产的相关性

Relationship between chemokine receptors CCR3,CCR5,CXCR3 and spontaneous abortion

目的探讨外周血、脾脏、胸腺趋化因子受体CCR3、CCR5、CXCR3与自然流产的关系。方法用双标记流式细胞分析技术检测自然流产模型组小鼠(CBA/J×DBA/2,n=14)、正常妊娠模型组小鼠(CBA/J×BALB/c,n=13)和正常非孕组小鼠(CBA/J,n=11)外周血、脾脏以及胸腺中CD4+T细胞CCR3、CCR5和CXCR3这三类趋化因子受体的表达。结果自然流产模型组外周血CD4+T细胞CCR3的表达率低于正常妊娠模型组(P<0.01),CCR5和CXCR3高于正常妊娠模型组(P<0.05,P<0.01);但三个指标与正常非孕组相比,差异均无统计学意义(P>0.05)。自然流产模型组脾脏CD4+T细胞CCR3的表达率低于正常妊娠模型组(P<0.01),高于正常非孕组(P<0.05);而CCR5和CXCR3高于正常妊娠模型组(P<0.05),但与正常非孕组相比,差异无统计学意义(P>0.05)。自然流产模型组胸腺CD4+T细胞CCR3的表达率低于正常妊娠模型组(P<0.05),高于正常非孕组(P<0.01);而CXCR3的表达率高于正常妊娠模型组(P<0.05),但与正常非孕组相比,差异无统计学意义(P>0.05);CCR5与其他两组相比,差异无统计学意义(P>0.05)。结论CD4+T细胞上CCR3、CCR5和CXCR3的表达异常可能在自然流产的发病中起作用。

Objective To explore the relationship between chemokine receptors CCR3, CCR5 and CXCR3 in peripheral blood, spleen and thymus and spontaneous abortion. Methods The expression of chemokine receptors CCR3, CCR5 and CXCR3 in CD4^＋ T cells in peripheral blood, spleen and thymus was detected by double-label flow cytometry. The samples were taken from the mice of spontaneous abortion model group （ CBA/J × DBA/2, n = 14） , normal pregnant model group （CBA/J ×BALB/c, n=13） and normal non-pregnant group（CBA/J, n=11）. Results The expression of CCR3 in CD4 ^＋ T cells in peripheral blood was lower in spontaneous abortion model group than that in normal pregnant model group （P 〈0.01）. The expression of CCR5 and CXCR3 in peripheral blood was higher in spontaneous abortion model group than that in normal pregnant model group （ P 〈 0.05, P 〈 0.01 ）. There was no significant difference on CCR3, CCR5 and CXCR3 between spontaneous abortion model group and normal non-pregnant group （P 〉 0.05）. The expression of CCR3 in CD4^＋ T cells in spleen in spontaneous abortion model group was lower than that in normal pregnant model group （P 〈 0.01 ） , but higher than that in normal non-pregnant group（P 〈 0.05）. The expression of CCR5 and CXCR3 was higher in spontaneous abortion model group than that in normal pregnant model group （ P 〈 0.05 ） , while there was no significant difference between spontaneous abortion model group and normal non-pregnant group （P 〉0.05）. The expression of CCR3 in CD4^＋ T cells in thymus in spontaneous abortion model group was lower than that in normal pregnant model group （ P 〈 0.05）, but higher than that in normal non-pregnant group （P 〈 0. 01 ）. The expression of CXCR3 was higher in spontaneous abortion model group than that in normal pregnant model group （ P 〈 0.05） , while there was no significant difference between spontaneous abortion model group and normal non-pregnant group （P 〉 0.05）. There was no significant difference in the expression of CCR5 in thymus among the three groups （ P 〉 0. 05）. Conclusion The abnormal expression of CCR3, CCR5 and CXCR3 in CD4^＋ T cells may play a role in the pathogenesis of spontaneous abortion.