心钠素对小鼠肿瘤生长和血管新生的抑制作用

Atrial natriuretic peptide inhibits growth and angiogenesis of tumor in mice

目的研究心钠素(ANP)的抗肿瘤作用及其在血管新生中的作用。方法通过微量泵将ANP输入带瘤小鼠,测量肿瘤体积,并用苏木素和靛红染色检验肿瘤中的血管新生。在基膜基质Matrigel上加入人脐静脉内皮细胞(HUVEC),使之形成毛细血管样结构,检测ANP对血管内皮细胞生长因子(VEGF165)刺激的HUVEC生长的影响。结果与对照组相比,ANP能抑制小鼠体内的黑色素瘤和肥大细胞瘤生长和血管新生(P<0.01,P<0.001),并抑制VEGF165诱导的HUVEC在基膜基质上形成毛细血管样结构(P<0.001)。结论ANP可能作为一种内源性血管抑制剂,抑制肿瘤生长及血管形成。

Objective To investigate the anti-tumor effect of atrial natriuretic peptide （ANP and its role in tumor angiogenesis. Methods ANP was infused into tumor-bearing mice by micro pump, then tumor size was measured and tumor angiogenesis was analysed by HE staining. The human umbilical vein endothelial cells （HUVEC） were placed in wells containing growth factor-reduced Matrigel in the presence or absence of vascular endothelial growth factor （ VEGF165） and ANP. Endothelial tube formation was observed. Results ANP inhibited growth and angiogenesis of melanoma and mastocytoma in the mice （ P 〈 0.01, P 〈 0. 001 ）. ANP inhibited VEGF165-induced tube formation on Matrigel（ P 〈 0. 001 ）. Conclusion ANP might have the potential clinical usefulness, as an endogenous angiogenic inhibitor, in inhibiting the growth and angiogenesis of tumor.