摘要
目的:应用微核试验(Micronucleus test,MCNT)技术研究小枣汁对环磷酰胺(CP)的抗突变作用的影响。方法:使用蚕豆根尖细胞微核技术进行微核率检测。结果:结果表明,不同浓度的小枣汁对环磷酰胺诱发的蚕豆根尖细胞微核有明显的抑制作用,1号-阳性和阴性对照试验组的微核率分别为52‰、30‰、26‰1、6‰、10‰、9‰7、0‰、7‰,小枣汁使CP诱发的微核率从52.0±5.3‰降低至9.7±2.5(P<0.001)。结论:说明小枣汁对CP诱发的遗传损伤具有明显的修复、保护以及抗突变作用。
Objective： Study the influence of the Chinese date juice to the anti - mutation role of cyclophosphamide （CP） by micronucleus experiment. Method： Make out a micronucleus rate examination by using the broad bean root- fine- cell＇s micronucleus technique. Result： The result indicated that Chinese date juice of differem concentrations has obvious inhibitory effects to the CP - induced broad bean root - fine - cell micronucleus. The mieronueleus rate of No. 1 - comparative test group is 5‰ , 30‰ , 26‰ , 16‰ , 10‰ , 9‰, 70‰, 7‰, respectively. Chinese date juice decreased the CP- induced micronucleus rate from 52.0± 5.3‰ to 9.7 ± 2.5 （P 〈 0.001）. Conclusion： The result showed that the Chinese date juice has an obvious repairing, protect and anti - mutate function to the CP- induced inherited damage.
出处
《生物技术》
CAS
CSCD
2008年第6期53-54,共2页
Biotechnology
基金
中国科学院心理研究所合作项目资助(No.140696)
关键词
微核率
抗突变效应
小枣
蚕豆根尖细胞
mieronueleus
anti - mutaiton effect
Chinese date
broad bean root - fine - cell
anti - mutation
