胃腺癌中PTEN的表达与IGF Ⅰ R的相关性

Expression and Relativity between Tumor Suppressor Gene PTEN and IGF ⅠR in Gastric Adenocarcinoma

目的研究抑癌基因PTEN和胰岛素样生长因子Ⅰ受体(IGF Ⅰ R)在胃腺癌组织中的表达,探讨PTEN与IGF Ⅰ R的相关性及其与胃癌临床病理学特征的关系。方法采用免疫组织化学SP法检测60例胃腺癌患者肿瘤组织和癌旁正常组织中PTEN和IGF Ⅰ R的表达水平。结果60例胃腺癌组织中PTEN蛋白表达阳性率仅为41.7%,明显低于癌旁正常组织,两者差异有统计学意义(P=0.001);其表达水平与年龄、性别无关(P>0.05),与分化程度(P=0.009)、肿瘤浸润程度(P=0.001)、淋巴结转移(P=0.004)和临床分期(P=0.002)显著相关;在胃腺癌组织中IGF Ⅰ R和PTEN蛋白表达之间呈明显负相关(rs=-0.469P<0.001)。结论抑癌基因PTEN的失活与胃癌的生物学行为密切相关;PTEN可能对IGF Ⅰ R的表达有着调控作用,联合检测两者可作为胃癌诊断、治疗和预后判断的参考指标。

Objective To study the expression between tumor suppressor gene PTEN and IGF Ⅰ R in gastric adenocarcinoma, and to investigate the relationship between PTEN and IGF Ⅰ R and their connection with clinical pathological characteristics. Methods The expression level of PTEN and ICF Ⅰ R was detected by immunohisto chemical SP technique in tumor and adjacent normal tissues of 60 patients with gastric adenocarcinoma. Results The positive rate of PTEN in tissues of 60 cases with gastric adnocarcinoma was 47. 1 %, which was significantly lower than that in adjacent normal tissues （P = 0. 001 ）. The expression level of PTEN had no correlation with age and sex （P〉0. 05）but it was significantly related to the extent of histological differentiation （P = 0. 009）, the depth of tumor invasion （P = 0. 001）, lymph node metastasis （P = 0. 004） and clinical stage （P = 0. 002）. There was significantly negative correlation between PTEN and IGF Ⅰ R expression （rs = - 0. 469, P〈0.001）. Conclusion The lost activity of tumor suppressor PTEN was closely related to the biological behavior of carcinoma of stomach. PTEN may control the expression of IGF Ⅰ R. PTEN and IGF Ⅰ R can serve as the markers in the diagnosis, treatment and prognosis of gastric adenocarcinoma.