调肝颗粒剂对大鼠人血白蛋白肝纤维化模型的治疗作用

Effect of pellet for regulating liver on treatment of liver fibrosis rats

目的:观察调肝颗粒剂对大鼠人血白蛋白肝纤维化模型治疗作用。方法:将85只大鼠分为正常对照组、模型组、调肝颗粒大、中、小3个剂量组(即观察组)和鳖甲软肝片对照组。人血白蛋白免疫致敏法建立大鼠肝纤维化模型,观察组大鼠分别灌服调肝颗粒3g·kg-1·d-1、1.5g·kg-1·d-1、0.75g·kg-1·d-1,1次/d,鳖甲软肝片对照组每日灌服鳖甲软肝片0.7g·kg-1·d-1,1次/d,治疗给药均在模型制备完毕后给药1个月药,股动脉取血,测定血清HA、PⅢP、Ⅳ-C和LN,取肝组织,Masson染色,并用MPEAS-500型图像分析仪进行图像分析。结果:治疗后,3个观察组HA含量均比模型组低,其中中剂量组与模型组比较差异有统计学意义(P=0.012);Ⅳ-C含量3个观察组均比模型组低,差异有统计学意义(P=0.001);PⅢP、LN含量3个观察组均比模型组低,但差异尚无统计学意义(P>0.05)。胶原相对含量3个治疗组均比模型组低,差异有统计学意义(P<0.01);以上指标,在3个观察组与鳖甲软肝片对照组之间比较,差异均无统计学意义。肝组织纤维化病理表现,在3个观察组与模型组之间比较,均有不同程度的改善。结论:调肝颗粒剂能降低人血白蛋白诱发免疫损伤性肝纤维化大鼠血清HA、PⅢP、LN和Ⅳ型胶原含量,并能改善大鼠肝脏的纤维化病理状况。

Objective： To explore the mechanism of effect of ＂ the pellet for regulating liver＂ on treating of liver fibrosis. Methods： The albumin-induced model of liver fibrosis was established in the rats to observe the effect of ＂the pellet for regulating liver＂ on treating liver fibrosis. First, choosing ninety male and female Wistar rats half and half; second, separating the rats into five groups, the control group, the model group, the group of Biejia Ruangan tablet, three observer groups （ the high-dose group, the sino-dose group and the low-dose group of ＂the pellet for regulating liver＂ ）, third, separately dealing with them relevantly; finally, observing the content of HA, P Ⅲ P, Ⅳ-C and LN in serum, the relative content of collagen of the liver pathology, and the liver pathology with biasson of model rats of each groups. Results： Compared with model group the content of HA in the three observer groups were more lower, and the sino-dose group of ＂the pellet for regulating liver＂ advanced was significant （P = 0. 012）, the content of Ⅳ-C in the three observer groups advanced were significant （ p = 0. 000）, the content of PⅢP and LN in the three observer groups advanced were no significant （ P 〉 0. 05 ） . The relative content of collagen advanced were significant （P 〈0. 01 ） . Compared with the model group the liver fibrosis of the three observer groups were varying degrees of improvement. Comparing with the group of Biejia Ruangan tablet, all indicators above advanced were no significant （P 〉 0.05） . Conclusion： ＂The pellet for regulating liver＂ can lower the content of HA and Ⅳ-C of the model rats, alleviate the degree of liver fibrosis.