麝黄消瘤方对小鼠肝癌血管生成的抑制作用

Inhibitory effect of Shehuang Xiaoliu Fang on hepatocarcinoma angiogenesis in mice

目的:观察麝黄消瘤方(SHXLF)对肝癌H22模型小鼠癌细胞侵袭和转移的影响,探讨其作用机制。方法:以小鼠H22肝癌淋巴道转移模型为对象,观察其淋巴结指数、脾指数、胸腺指数,光镜下观察肝癌H22细胞局部侵袭和淋巴结转移程度。免疫组化SP法观察局部瘤组织血管内皮细胞生长因子(VEGF)表达水平和微血管密度(MVD)。结果:麝黄消瘤方可使局部瘤组织的侵袭和腘窝淋巴结转移程度及淋巴结指数有降低趋势;大中剂量组可使胸腺指数明显升高(P<0.05)。中药各治疗组可明显降低肝癌组织VEGF表达水平和MVD(P<0.05)。结论:SHXLF可抑制H22肝癌小鼠癌组织在局部及淋巴结的侵袭转移程度。其作用机制可能是通过调整免疫功能,杀伤癌细胞,抑制肿瘤血管生成(降低VEGF和MVD)而实现的。

Objective： To study the effect of Shehuang Xiaoliu Fang （SHXLF） on treatment of invasion and metastasis in mice with transplanted liver cancer, and to research the possible mechanism. Methods： Observe the index of lymph nodes, thymuses, spleens of the mice （SPF） which were transplanted liver cancer （ H22 ） subcutaneously into left footpads to be estab- lished as experimental lymphatic metastasis models, the invasion and metastasis occurring in footpads and lymph nodes, and the expression of vascular endothelial growth factor （VEGF） , the microvaseular density （MVD） in tumor tissues were measured by immunohistochemistry staining. Results： The SHXLF treatment groups could decrease the level of invasion and metastasis in footpads and popliteal fosaa lymph nodes of hepatocellular carcinoma, the index of lymph, and the high dose group （group D） were significantly decreased （P 〈 0. 05 ） . The SHXLF middle, high dose groups （group C, D） could significantly increase index of thymus （ P 〈 O. 05 ） . On the other hand, the group B, C, D could significantly decrease the VEGF content and MVD. Conclusion ： The SHXLF can inhibit invasion and lymphatic metastasis of liver tumor in the experimental mice by improving immune functions, killing the carcinoma cell, and decreasing the expression of VEGF and MVD.