摘要
采用微电极胞内穿刺技术探查鲫鱼Mauthner细胞(M-细胞)对迷叶刺激的电反应特征。电刺激鲫鱼迷叶背面中央外侧区,可在双侧M-细胞胞体,腹侧树突和外侧树突近端记录到一种复合性兴奋性突触后电位(迷叶诱发性EPSP)。迷叶诱发性EPSP表现较短潜伏期(0.584±0.16 ms),较长持续时间(6.20±2.13 ms),幅度分级和刺激频率依从等特征,并可引起M-细胞顺向激活。多点胞内连续穿刺实验显示迷叶诱发性EPSP起源于腹侧树突远端。实验结果提示,迷叶—M-细胞通路可能包含一组长短不等的神经元链,它们依链的短或长,由近及远依次投射在腹侧树突的限定节段。
The characteristics of the electrical responses to stimulation of the vagal lobe in c were explored with microeletrode intracellular recording technique. A composite excitatory po rucian carp Mauthner cell stsynaptic potential （vagal lobe-evoked EPSP）could be induced on the soma,the ventral dendrite and the proximal end of the lateral dendrite in crucian carp Mauthner cell（M-cell） at either side by stimulation of the medial-lateral region of the vagal lobe dorsal surface. The vagal lobe-evoked EPSP presents the characteristics of relative short latency （0.58 ± 0.16 ms）, longer duration（6.20 ± 2. 13 ms）, graded amplitude and dependence on stimulation frequency, and always activates the M-cell orthodromically. Multiple intracellular recording shows that the vagal lobe-evoked EPSP is originated in the distal end of the ventral dendrite. The results suggest that the vagal lobe-M-cell pathways are probably composed of a group of neuron chains with different numbers of synaptic relays and they are projected to the restricted regions of the ventral dendrite in order of length of the chains.
出处
《实验室研究与探索》
CAS
2008年第12期37-41,共5页
Research and Exploration In Laboratory
基金
北京市教委基金(KM200710025004)
