微小RNA在前列腺癌组织中的表达及意义

THE EXPRESSION AND SIGNIFICANCE OF MICRORNAS IN PROSTATE CANCER

目的研究5种微小RNA(microRNA)在前列腺癌组织中的表达情况及其临床意义,并探讨它们在前列腺癌诊断中的潜在应用价值。方法应用核酸分子原位杂交(ISH)的方法,结合组织芯片(TMA)技术检测38例良性前列腺增生(BPH),52例前列腺癌(PCa)及2例正常前列腺组织中5种miRNAs的表达情况。结果(1)miR-96、miR-183、miR-139在PCa中的表达率分别为67.31%(35/52),71.15%(37/52),67.31%(35/52),分别与它们在BPH中的表达率44.74%(17/38),47.37%(18/38),39.47%(15/38)相比,差异有统计学意义(P<0.05);miR-145在PCa中的表达率为21.15%(11/52),较BPH的63.16%(24/38)低(P<0.01);miR-let-7d在PCa中的表达率为25.00%(13/52),较BPH的34.21%(13/38)无显著性差异(P>0.05)。(2)5种miRNAs的表达与前列腺癌患者的年龄及血清PSA水平均无明显相关性(P>0.05);5种miRNAs表达与肿瘤的Gleason评分均相关(P<0.01);miR-96、miR-let-7d及miR-139的表达与肿瘤的临床分期有关(P<0.01),miR-145、miR-183的表达则与其无明显相关性(P>0.05);miR-96的表达与肿瘤累及前列腺的叶数相关(P<0.01),而其余四种miRNAs的表达则与其无明显相关性(P>0.05)。结论miRNAs与前列腺癌的发生有关,并可能作为前列腺癌早期诊断及预后评估的重要生物标记物。

Objective To research the expression of five individual miRNAs and their significance in prostate cancer, and to discuss their potential use for the diagnosis of prostate cancer. Methods In situ hybridization （ISH） staining combined with tissue microarray technology for five miRNAs was performed in 38 samples of benign prostate hyperplasia （BPH）, 52 samples of prostate cancer （PCa） and 2 samples of normal prostate tissue. Results （1） The positive rates of miR-96, miR-183 and miR-139 in PCa were 67.31% （35/52）, 71.15% （37/52）, and 67.31% （35/52）, respectively. As compared with their expression levels in BPH, which were44.74% （17/38）, 47.37% （18/38）and 39.47% （15/38）, respectively, there were significant differences between BPH and PCa （P〈0.05） . The positive rate of miR-145 was lower in PCa （21.15%, 11/52） than that in BPH （63.16%, 24/38） （P〈0.01） . There was no significant difference in the positive rate of miR-let-7d between BPH （34. 21%, 13/38） and PCa （25.00%, 13/52） （P〈0.05） . （2） The expression of these five miRNAs correlated with Gleason Grading of prostate cancer （P〈0.01）, but not with age and serous PSA level of the patient （P〉0. 05） . On the other hand, the expression of miR-96, miR-let-7d and miR-139 correlated with tumor clinical stage （P〈0. 01）, but there was no significant correlation between the expression of the other two miRNAs and tumor clinical stage （P〉0.05） . The expression of miR-96 correlated with the extension of tumor invasion in prostate （ P〈0. 01）, but the remaining four miRNAs did not correlate with that （P〉0.05）. Conclusion The miRNAs are involved in the development of prostate cancer, and they might be important biomarkers for early diagnosis and prognostic assessment of prostate cancer.