膜乳化—复乳化法制备载蛋白高分子微球

Preparation of protein loaded polymer microspheres by membrane emulsification by multiple emulsification technique

选择乙基纤维素(EC)为微球材料,牛血红蛋白(Hb)为模型药物,采用膜乳化—复乳(W1/O/W2)法制备了载蛋白高分子微球。采用扫描电子显微镜(SEM)考察微球形态及内部结构,激光粒度仪测定微球粒径及粒径分布。结果表明,膜孔径是决定微球粒径的主要因素,微球粒径随EC浓度和初乳相体积分数增大而增大。随着初乳相体积分数的增大,微球表面微孔数目减少,但孔径增大。当操作压力稍大于膜乳化初始临界压力时,可制得粒径单分散的载蛋白高分子微球。这一结果对制备粒径单分散的载蛋白高分子微球具有一定的参考价值。

The protein loaded polymer microspheres are prepared by using membrane emulsification - multiple emulsification（W1/O/W2）technique using ethyl cellulose （EC） as microsphere materials and hemoglobin （Hb） as model drug. The morphology and internal structure of microspheres are observed by means of scanning electron microscopy （SEM）, the size and its distribution of polymer particles are determined by laser particle sizer. The results shows that the main factor determining microsphere size is membrane pore size. the size of microspheres increases with the increasing of EC concentration and the volume of colostrum phase. With the increasing of the volume of colostrum phase, pores on the surface of particles decreases, but the size of the pore increases. When the operating pressure is slightly above the critical pressure in the beginning of the membrane emulsification, the monodisperse protein loaded polymer microspheres can be prepared. This result has the certain reference value to the preparation of monodisperse protein loaded polymer microspheres.