毛细管区带电泳法测定杜仲药材中绿原酸的含量

Determination of chlorogenic acid in Cortex Eucommiae by capillary zone electrophoresis

目的:建立采用高效毛细管电泳测定杜仲中有效成分绿原酸(CA)含量的方法,比较不同产地杜仲中绿原酸含量。方法:采用毛细管区带电泳(CZE),以内标法测定:运行电解质为含80mmol·L-1硼砂缓冲液-100mmol·L-1氢氧化钠(20∶1,pH=9.32),工作电压30kV,柱温25℃,检测波长为340nm,内标物为盐酸小檗碱。结果:绿原酸在0.006～0.574mg·mL-1的浓度范围内呈良好的线性关系(r=0.9994),平均回收率为101.43%,RSD为1.44%。结论:该方法简便、准确,重复性好。

Objective： To establish a capillary zone electrophoresis （CZE） method for the determination of chlorogenic acid in Cortex Eucommiae and to compare the contents of chlorogenic acid in Cortex Eucommiae from different habitats.Methods： Capillary zone electrophoresis method was carried out in a buffer solution containing 80 mmol ·L^-1 borax and 100 mmol·L^-1 caustic soda （20： 1, pH = 9.32）. The applied voltage was 30 kV and the capillary temperature was 25 ℃. The detection wavelength was 340 nm and berberine hydrochloride was selected as a internal standard. Results： Chlorogenic acid showed a good linear relationship in the range of 0.006 - 0.574 mg· mL ^-1 （ r = 0.999 4）. The average recovery was 101.43% and RSD was 1.44%. Conclusion： The method is simple, accurate and well repeatable.