锰处理大鼠PC12细胞的增殖抑制及JNK1/2活化表达的变化

Changes of proliferation and activity expression of JNk1/2 of PC12 cells treated by manganese

目的探讨氯化锰（MnCl2）对嗜铬细胞瘤（PC12）细胞增殖的抑制作用及对c—Jun氨基端激酶1／2（JNKI／2）磷酸化活化表达量的变化。方法大鼠PC12细胞在含0—500μmol／L浓度Mncl2的培养基中培养3d后，细胞计数法观察MnCl2对细胞增殖的抑制率。Western blot检测JNK1／2磷酸化及总JNK 1／2表达量。结果MnCh对PC12细胞的增殖有显著的抑制作用，且呈剂量一效应关系。随着MnCh浓度的增加，JNK1／2磷酸化水平逐渐升高，蛋白表达水平分别比对照组升高了1．91、2．86、4．13倍（P〈0．05）。结论．MnCl2对PC12细胞的增殖有显著的抑制作用，而上调p-JNK1／2的表达。

Objective To explore the changes of proliferation and activiaty expression of c-Jun N-terminal kinase1/2（JNK1/2） of pheochromocytoma cells treated by manganese in vitro. Methods PC12 cells were exposed to MnCl2 at the doses of 0 - 500 μmol/L for 3 days. The inhibition of the cells was determined by cell counting, and the perphesphorylation and activity expression of c-Jun N- terminal kinase1/2（JNK1/2） by Western blot. Results MnC12 inhibited the proliferation of PC12 cells in a dose dependent manner. Western blot test showed that MnCl2 increased the perphesphorylation and activity expression of c-Jun N-terminal kinase1/2（JNK1/2） in a dose-dependent manner （P 〈 0.05 ）. Conclution The inhibition of the proliferation of PC12 cells induced by MnCl2 could be association with up-regulating p-JNK1/2 expression.