FK506和环孢霉素A在兔角膜缘移植术后抑制免疫排斥反应的疗效比较

The Applications of Immunosuppressant FK506 and Ciclosporin A on Limbal Allograft Transplantation in Rabbits

目的研究FK506和环孢霉素A对同种异体兔角膜缘移植术后免疫排斥反应的抑制效应。方法将30只有角膜缘干细胞缺陷症的新西兰白兔随机分成3组:FK506治疗组、环孢霉素A治疗组和对照组,同种异体角膜缘移植术后分别给予0.5%FK506滴眼液、1%环孢霉素A滴眼液和生理盐水点眼,用药5周。术后持续观察11周,以角膜缘植片水肿、混浊程度、排斥反应发生时间和角膜新生血管作为眼表评估指标。结果观察期内,FK506组在抑制新生血管指数方面低于环孢霉素A组(P<0.05),而两组在上皮排斥反应发生时间、移植排斥指数方面无显著性差异(P>0.05)。结论同种异体角膜缘移植术后早期应用0.5%FK506和1%的环孢霉素A滴眼液均可有效抑制免疫排斥反应,在抑制角膜新生血管发生方面,FK506的作用强于环孢霉素A。

Objective To study the effect of immunosuppressent FK506 on limbal transplantation ejectionmodel in rabbits.Methods Thirty New Zealand rabbits （30 eyes） underwent corneal epithelial debfidementand limbal ablation to estiblish limbal stem cell deficiency model and were randomized into the follow three groups, which were treated by limbal allogrft transplantation. After transplantation, FK506 group was treated by FK506 eyedrops （0.5 mg/ ml）, while poitive control group by 1% CsA eye droups and untreated group by physiological saline for 5 weeks. Grafts survival and ocular surface was inspected clinically for 11 weeks. Results Epithelium rejection appeared firsdy in the untreated group, CsA groupeame second, FK506 group appeared epithelium rejection late. Conclusion Early FK506 eyedrops after operation can suppress, the immunity rejection reaction effeetively which is less than in CsA group. Also it can inhibit the growth of new blood vessels.