白假丝酵母菌DNA提取方法的研究

A Method of DNA Extraction from Saccharomyces albicans

目的建立一种提取白假丝酵母菌DNA的有效方法。方法采用蜗牛酶消化破壁形成原生质体,饱和酚/氯仿抽提法提取基因组DNA,用琼脂糖凝胶电泳及PCR反应等进行鉴定。结果提取物经琼脂糖凝胶电泳检测到DNA主带,基本无DNA碎带,提取的DNA浓度为(1.18±0.36)μg/μl,纯度好(OD260/OD280>1.7),不用RNase酶处理,无需任何纯化即可用于PCR扩增。结论本研究中提取DNA的方法简便易行,提取物可适用于各种分子生物学研究。

Objective To establish an affective method of DNA extraction from S. albicans. Method The protoplast was formed by using glusulase to digest the fungal cell well. The genomic DNA was extracted by phenolchloroform. The quality was checked by agarose gel electrophoresis and polymerase chain reaction. Result The extracted products have a major DNA band, which can be observed through agarose gel electrophoresis. A little DNA fragments can be seen. The concentration of DNA extraction was （1.18±0.36）ug/uL with high purity （OD260/OD280〉 1.7）. The DNA can be directly used in PCR amplification without RNase digestion to remove RNA and any further purification. Conclusion We explore a convenient method of extracting DNA, which could be used for various molecular biology experiments.