p53在肺癌中的表达及其与VEGF和血管新生关系的研究

The Study of p53 Protein Expression and Their Relationship with VEGF and Angiogenesis in Human Lung Cancer

目的探讨63例人肺癌中p53蛋白的表达与血管内皮细胞生长因子(VEGF)和血管新生的关系。方法采用免疫组织化学方法,以抗CD34单抗标记血管内皮细胞以测定血管新生,抗p53多抗标记p53蛋白,抗VEGF165单克隆抗体检测VEGF的表达。结果所有肺癌组织均有不同程度的血管新生,iMVD4～138.7(44.5±29)/×400,p53突变率为54%(34/63),VEGF的表达率为50.8%(32/63)。p53突变率与组织类型、临床分期、病人性别、年龄等临床参数无关;VEGF与组织类型、临床分期、病人性别、年龄等临床参数无关。结论p53蛋白表达与VEGF和血管新生未见相关;人肺癌的发生发展与p53蛋白过度表达有关。

Objective To evaluate the expression of p53 and their relationship with VEGF and angiogenesis in human lung cancer. Method Expression of p53, VEGF and angiogenesis in 63 cases of human lung cancer were examined using immunohistochemical methods.Monoclonal antibody against CD34 was used to identify intra-tumoral micro-vessel density （iMVD）. Anti-VEGF^165-Ab3 was used to detect VEGF expression and anti-p53 pAb was used for p53 expression. Result Angiogenesis was observed in all lung cancer samples and iMVD was found to vary from 4 to 138.7 （44.5±29）/×400. p53 protein immuno-reactivity was detected in 34 out of 63 cases （54%）.Positive staining for VEGF was observed in 32 of the 63 cases （50.8%）. Frequency of p53 mutation and VEGF expression were independent on their TNM stages, sex and age. Regression analysis showed no correlation between the iMVD and the expression of VEGF and p53. Conclusion There was no association between p53 expression and VEGF and iMVD. There was a correlation between an overexpression of p53 and the development of lung cancer.