大鼠肌卫星细胞移植对失神经骨骼肌张力的影响

The Effect on Tension of Denervated Skeletal by Transplantation of Skeletal Muscle Satellite Cell in Rats

目的探讨大鼠肌卫星细胞移植能否延缓失神经骨骼肌萎缩。方法将16只成年Wistar大鼠分为实验组与对照组,两组均切断大鼠右后肢胫神经,建立腓肠肌失神经动物模型。实验组:将体外培养的同种异体肌卫星细胞悬液注射到失神经腓肠肌内;对照组:则注射等量的生理盐水于相同部位。术后第4周,采用电刺激方法,检测失神经骨骼肌的张力变化情况。结果实验组与对照组相比,失神经腓肠肌收缩张力的差异有统计学意义。结论本实验表明将肌卫星细胞异体移植到失神经骨骼肌内可明显延缓骨骼肌的萎缩进程,为再生神经到达靶器官提供较多的时间,进而为解决再生神经延伸到靶器官前,靶器官已发生不可逆性萎缩,严重制约再生神经效果的临床难题提供一个新的研究思路。

Objective To explore if the muscle satellite cells in rats can delay the skeleton muscular dystrophy. Method Sixteen male Wistar rats randomly assigned into the experimental and the control group.The tibial nerve of the right leg of each rat was cut with lcm lesion, and the model of denervated skeletal muscle was made, Equal amount of muscle satellite cells suspension or physiological saline was injected into the denervated gastrocnemius of the experimental group and the control group respectively. Four weeks after transplantation,both groups were stimulated with different strength of electric impulses to measure the lension of denervated skeletal muscle. Result The differences of tension of skeletal muscle between two groups was statistically significant. Conclusion The transplantation of rat muscle satellite cells into denervated muscle can maintain the muscular tension, and thus alleviate the rate of atrophy of skeleton muscle, and help the recovery of the muscle＇s function. This may provide a clinical effective way to prevent and treat denervated skeleton muscular dystrophy.