大鼠显微注射胚胎采集方法的比较

Comparative Study on Different Embryo Collecting Method for Rat Microinjection

目的优化大鼠显微注射前胚胎采集的方法。方法选用最佳超排5周龄SD大鼠,腹腔注射PMSG-HCG,分别用撕开输卵管壶腹部和输卵管伞端冲洗两种方法采集胚胎,比较获得卵数,受精率,注射存活率等的差异。结果冲洗输卵管伞端采集胚胎的方法所得到的受精率、存活率都较高,与撕开输卵管壶腹部方法比较差异显著(P<0.01),胚胎总数无显著性差异(P>0.05)。结论冲洗输卵管伞端采集胚胎的方法为大鼠显微注射前胚胎采集比较好的方法。

Objective To improve embryo collecting method for rat microinjection. Methods PMSG- HCG（ip） were injected into 5 weeks SD rats. Embryos were collected by tearing intumescences of oviduct or clysising fimbriae tubae. The amount of embryos, rates of fertilization, and survival rate of embryos after injection were analyzed to compare the two methods. Results The amount of embryos, rates of fertilization, and survival rates of embryos after injection of the embryos collected by clysising fimbriae tubae were higher than those collected by tearing intumescence of oviduct. There are significant differences between the two methods. Conclusion Clysising fimbriae tubae is a better embryo collecting method for rat microinjection.