摘要
目的进一步探讨心脏衰老的机制及干预因素的影响。方法将30只20月龄的Wistar大鼠随机分为观察1组、观察2组和老年对照组各10只,设10只3月龄大鼠为青年组。观察1组、观察2组分别予阿托伐他汀1、10 mg/(kg.d)灌胃,青年组和老年对照组予等体积生理盐水灌胃,均连续4个月。采用RT-PCR方法检测各组心肌过氧化物酶体增殖物激活物受体α(PPARα)mRNA和白细胞介素(IL)-1βmRNA表达水平。结果与青年对照组比较,老年对照组PPARαmRNA表达水平显著降低(P<0.01),IL-1βmRNA表达水平显著增高(P<0.01);与老年对照组比较,观察1组和观察2组PPARαmRNA表达水平显著升高(P<0.01),IL-1βmRNA表达水平显著降低(P<0.01),尤以观察2组为著。结论老年大鼠心肌IL-1βmRNA表达显著增高、PPARαmRNA表达显著降低,此可能在心脏衰老发生、发展过程中起重要作用;阿托伐他汀可通过激活PPARα抑制IL-1β异常表达,此可能为其对抗衰老、改善心脏功能的重要机制之一。
Objective To investigate the changes of the expression of PPARα mRNA and IL-1β mRNA in myocardium of aging tars and the effects of atorvastatin on it. Methods 30 Wister rats at 20 month old were divided into three groups： aging control group, observed group one[10 mg（kg·d） ], observed group two[1 mg（kg · d） ]. Another 10 Wister rats at 3 months old were selected as young control group, atorvastatin was delivered by intragastric administration to observed groups for 4 months. Normal sodium was delivered to control group by intragastric administration too. The expression of PPARα mRNA and IL-1β mRNA were evalulated by RT-PCR. Results （1）The expression level of PPARα mRNA in aging control group was significantly lower than that in the young control group（P 〈 0.01 ）, atorvastatin upregulated PPARα mRNA expression in a dosage-dependent manner （ P 〈 0.05）. （2）The expression level of IL-1β mRNA in aging control group was significantly higher than that in young control group（P 〈 0.01 ）, atorvastatin inhibited IL-1β mRNA expression in a dosage-dependent manner （ P 〈 0.05）. Conclusion Atorvastatin can inhibit the expression of IL-1β mRNA in myocardium of aging rats by upregulating the expression level of PPARα mRNA, which may be the mechanism of myocardium protection.
出处
《山东医药》
CAS
北大核心
2008年第35期16-18,共3页
Shandong Medical Journal
基金
北京市自然科学基金资助项目(7082083)
