Maspin在宫颈癌组织中的表达及意义

Expression of Maspin in cervical carcinoma and its clinical significance

目的进一步探讨Maspin的抑癌机制。方法应用免疫组化PV6000通用二步法检测Maspin在正常宫颈组织、宫颈上皮内瘤样病变(CIN)及宫颈癌组织中的表达并计数MVD,分析Maspin与MVD之间的相关性及二者与宫颈癌临床病理参数间的关系。结果Maspin在正常宫颈、CIN、宫颈癌组织中的表达率逐渐降低,MVD则逐渐升高(P均<0.05);Maspin在宫颈癌组织中的表达与FIGO分期、组织分化程度和淋巴结转移有关,与MVD呈显著负相关(P均<0.05)。结论Maspin可能通过减少肿瘤新生血管形成抑制宫颈癌的生长、浸润和转移。

Objective To explore the resisting mechanism of Maspin to cervical cance. Methods Parafin-embedded tissues from normal epithelium, cervical intraepithelial neoplasia （CIN） and cervical cancer were studied for Maspin expression by immunochemistry. Microvessel density （MVD） in cervical cancer was labeled using anti-CD34 antibody. Maspin expression was compared with clinical parameters and MVD of tumors. Results The positive rates of Maspin expression in normal epithelium, CIN and cervical carcinoma were 100.00%, 62.96% and 38.71%, respectively. And MVD of which increased graduslly （ P 〈 0.05 ）. Maspin expression showed a significantly negative association with FIGO staging,lymph node metastasis, differentiation （P〈0.05） and MVD （P 〈0.05）. Conclusions Maspin might inhibit tumor growth, metastasis and invasion via its anti-angiogenie effect.