双甘膦和草甘膦的高效液相色谱分析法

HPLC determination of glyosphate and phosphonomethyliminodiacetic acid

利用高效液相色谱,采用外标法测定样品中的草甘膦和双甘膦的含量,紫外检测器检测波长为195nm,色谱柱为 SAX 强阴离子交换柱,用0.005mol·L^(-1)磷酸二氢钾缓冲液(pH 2.0)为流动相,对样品进行分析,试验测得草甘膦和双甘膦的相关线性系数分别为0.9996和0.9989,标准偏差分别为0.035和0.098,回收率分别为98.5%～101.5%和97.8%～102.5%。

The determination of phosphonomethyliminodiacetic acid by external stardand method was described A solution of 0.005mol·L^-1 KH2PO4（pH 2.0） was used as the mobile phase, SAX column was used and the UV-detection at 195nm was applied. The correlatiom coefficient for both compounds were respectively found to be 0.9996 and 0.9989. The stardand deviation were respectively 0.035 and 0.098. Recoveries were respectively in the range 98.5% - 101.5% and 97.8% - 102.5%.