大鼠40%小体积肝移植模型建立及移植肝的病理观察

ESTABLISHMENT OF THE SMALL-FOR-SIZE LIVER TRANSPLANTATION MODEL AND INVESTIGATION OF THE HISTOPATHOLOGIC CHANGES OF THE GRAFTS IN RATS

目的建立稳定的大鼠40%小体积肝移植模型,并观察术后移植肝功能与病理的变化。方法采用改良二袖套法建立大鼠40%小体积肝移植模型,观察1w生存率,并于术后1、2、4、7天检测肝功能、移植肝组织学变化和免疫组化法检测肝细胞的增殖细胞核抗原(PCNA)。结果手术成功率97.5%,1w生存率60%,无肝期平均(12±2.5)min,丙氨酸转氨酶(ALT)、总胆红素(TB)术后即第1天即明显增高,第2天最高,以后逐渐降低。组织学检查术后2天即可见较多二倍体和多倍体肝细胞。免疫组化检测移植肝PCNA表达于术后第2天最高。结论通过技术改进,简化了手术操作,提高了手术成功率及模型的稳定性。大鼠小体积移植肝仍具有较强的再生能力。

Objective To establish and modify a rat model of small-for-size orthotopic liver transplantation and investigate the postoperative liver function and histopathologic changes of the grafts. Methods Modified two-cuff technique was applied to establish a rat model of 40% small-for-size orthotopic liver transplantation. One week survival rate, main indexes of liver function, histology and the expression of the proliferative cell nuclear antigen （PCNA） of liver graft by immunohistochemical method were detected on the 1 st, 2nd,4th and 7th day after transplantation, respectively. Results The successful rate of the operation was 97.5%. One week survival rate was 60%. The mean time of nonhepatie time was （12±2. 5 ） min. Alanine aminotransferase （ALT） and total bilirubin （TB） began to elevate from the first day and peaked on the second day after surgery. Histological findings indicated that more diploid and polyploid hepatocytes could be seen on the 2nd day after surgery. The expression of PCNA of liver graft peaked on the 2nd day after surgery. Conclusion The model is easily available and highly reproducible, and the stability of the model is inproved by modifying the technique. The graft of the rat has a great ability of regeneration after small-for -size liver transplantation.