迎红杜鹃组培快繁技术体系的研究

Study of the rapid propagation technic of Rhododendron mucronulamm Turcz by tissue culture

迎红杜鹃休眠枝水培外植体和野外采集当年生嫩枝的最适消毒时间分别为2,5 min,接种于pH值为5.0的改良MS+ZT 5.0 mg/L+NAA 0.05 mg/L+蔗糖30 g/L+琼脂粉6.5 g/L,培养基中效果最好,诱芽率达到76.67%;继代增殖培养效果最好的培养基为改良MS+ZT 1.0 mg/L+NAA 0.1 mg/L,增殖率和增加高度分别达到8.94%和1.88 cm;迎红杜鹃组培苗最佳生根条件为1/4改良MS+IBA 0.5 mg/L+蔗糖20 g/L,生根率达93%;炼苗移栽成活率最高的方法是先在河沙中锻炼10 d再移栽至松毛土∶腐植土∶河沙=1∶2∶1的基质中,成活率达88%.

The best disinfection time of water cultured explant and current shoot of Rh. mucronulamm Turcz were 2 min and 5 min. The best primary culture medium was improved MS＋ZT 5.0 mg/L＋ NAA 0.05 mg/L ＋ sucrose 30 g/L＋ agar 6.5 g/L（ pH is 5.0）, the differentiation rate was 87.45% ; subculture medium was improved MS＋ZT 1.0 mg/ L ＋ NAA 0.1 mg/L, shoot multiplication coefficient and increased height was 8.94 and 1.88 era, respectively; the best rooting conditions were 1/4 improved MS ＋ IBA 0.5 mg/L sucrose 20 g/L, rooting rate reached 93%; the best way for domestication was trans-planted in turfy soil ： humus soil ： river sand =1：2：1 after training in the sand for 10 days, the survival rate reached 92.45%.