采用组织芯片技术分析Ki-67抗原在胃癌中的表达及其意义

The expression of Ki-67 antigen and evaluation in gastric carcinoma

目的检测增殖细胞核抗原Ki-67抗原在胃癌中的表达情况，分析与胃癌病理特征之间的关系，探讨胃癌的发生、发展及相关淋巴结转移的关系。方法取胃癌标本共88例作为试验组，慢性浅表性胃炎标本16例作为对照组，通过组织芯片技术和免疫组织化学染色法确定检测部位。结果Ki-67抗原在胃癌中的表达明显高于慢性浅表性胃炎，二者的阳性率分别为89．4％和26．7％，P〈0．001，Ki-67抗原在有淋巴结转移的胃癌中的表达明显高于无淋巴结转移者，二者的阳性率分别为92．2％和62．5％，P=0．036。结论Ki-67抗原在胃癌中高表达，且与胃癌淋巴结转移相关，对胃癌预后有一定意义。

Objective To examine the expression of Ki-67 antigen in gastric carcinoma, and to evaluation the relationship between Ki-67 and clinical and pathological characters of gastric carcinoma and participate in the generation, development and metastasis of lymph node of gastric carcinoma. Methods Eighty-eight paraffin wax blocks of gastric carcinoma were divided into experiment group. The control group 16 cases of chronic superficial gastritis were collected from the samples of duodenum ulcer or gastric ulcer. Ki-67 antigen in gastric carcinoma and chronic superficial gastritis was detected by immunohistochemistry method and tissue microarray. Results The expression of Ki-67 antigen in gastric carcinoma was much higher than that in chronic superficial gastritis（89.4% and 26.7% respectively ,P 〈 0. 001 ）. The expression of Ki-67 antigen in gastric carcinoma with metastasis of lymph node was much higher than that without metastasis （92. 2% and 62. 5% respectively, P = 0. 036）. Conclusion Since the expression of Ki-67 antigen in gastric carcinoma is very high, and it correlates with metastasis of lymph node of gastrc carcinoma,it can serve as an important mark for the prognosis of gastric carcinoma.