银屑灵熏剂蒸汽治疗对寻常型银屑病外周血单个核细胞表达T-bet、GATA-3蛋白水平的影响

Effect of Fumigation Therapy with Yinxieling on T-bet and GATA-3 Protein Expression in the Peripheral Blood Monocyte of Patients with Psoriasis Vulgaris

目的探讨银屑灵熏剂蒸汽治疗寻常型银屑病的临床疗效及对外周血单个核细胞(PBMC)表达T-bet及GATA-3蛋白水平的影响。方法对30例寻常型银屑病患者行银屑灵熏剂蒸汽治疗,观察PASI评分下降率并使用Western blot法检测患者及25名健康对照者PBMC中T-bet和GATA-3蛋白水平的变化,观察PASI评分与T-bet和GATA-3蛋白水平相关性。结果30例患者治愈12例,显效9例,有效8例,无效1例。治愈率为40.0%,有效率为96.7%。寻常型银屑病患者PBMC表达T-bet蛋白水平(1.7917±0.3840)较对照组(0.8860±0.1486)升高(P<0.01)。GATA-3蛋白水平(0.8777±0.3114)较对照组(1.2384±0.1783)降低(P<0.01)。经银屑灵熏剂治疗后,T-bet蛋白表达降为1.3410±0.3642,而GATA-3蛋白升高为1.0883±0.2435,治疗前后改变均有统计学意义(P<0.01)。其中PASI评分与T-bet蛋白表达水平呈正相关(r=0.7448,P<0.01),与GATA-3蛋白表达水平呈负相关(r=-0.8291,P<0.01)。结论银屑灵熏剂蒸汽治疗银屑病有效,其可能机制是通过调节寻常型银屑病患者PBMC中转录因子T-bet/GATA-3蛋白的平衡表达,纠正寻常型银屑病患者Th1/Th2细胞亚群失衡的免疫异常,有效治疗银屑病。

Objective To evaluate the effect of fumigating with Yinxieling （YXL） in treating patients with psoriasis vulgaris and its influence on T-bet and GATA-3 protein expressions in peripheral blood monocyte （PB- MC）. Methods Western blot method was employed to detect the T-bet and GATA-3 protein expressions in PBMC of 30 psoriasis vulgaris patients before and after they received fumigation therapy with YXL, also in 25 healthy persons for controls. The therapeutic efficacy was observed and the relationship between PASI scores and levels of T- bet and GATA-3 analyzed. Results After treatment, 12 out of the 30 patients were cured, 9 were markedly effective, 8 effective and 1 unchanged, the cure rate being 40.0% and the effective rate 96.7%. Level of T-bet expression in PBMC of patients was 1. 7917 ± 0. 3840, which was higher than that of healthy persons （0. 8860 ± 0. 1486, P 〈0.01） , but the GATA3 expression was lower than that in control （0. 8777 ±0. 3114 vs 1. 2384 ±0. 1783, P 〈 0.01 ）. However, the two indexes were restored after fumigation to 1. 3410 ±0. 3642 and 1. 0883 ±0. 2435 respectively, showing significant difference to those before fumigation （P 〈 0.01 ）. Correlation analysis showed that PASI score was positively correlated with level of T-bet expression （ r = 0. 7448, P 〈 0.01 ） and negatively correlated with level of GATA-3 expression （r = -0. 8291, P 〈 O. O1 ）. Conclusion Fumigation therapy is effective in treating psoriasis vulgaris, its mechanism is possibly by way of modulating the equilibrium of the transcription factors T-bet and GATA-3 protein expressions in PBMC, and rectifying the immune abnormality of Thl/Th2 subsets imbalance.