凹陷蛋白-1基因在乙型肝炎相关性肝癌中的表达及其意义

Expression of caveolin-1 gene in hepatitis B-related hepatocellular carcinoma and the clinical significance thereof

目的探讨候选抑癌基因凹陷蛋白（Caveolin）-1在肝细胞癌（HCC）中的表达及其与HCC侵袭、转移的关系。方法对123例HCC标本以及MHCC-97L和MHCC-97H肝癌细胞株进行实时荧光PCR法、免疫组化法及Western印迹法检测Caveolin-1mRNA及蛋白表达，分析其表达与肿瘤侵袭、转移性间的关系。结果Caveolin-1在肝癌组织中较癌旁组织表达明显下调（P〈0．05）；HCC中的表达水平与大／小肝癌（≥5cm或〈5cm）、AFP表达水平、有无大血管侵袭、肿瘤单发或多发及pTNM分期5个因素均呈显著负相关（P〈0．05），其在MHCC-97H细胞株中的表达显著低于MHCC-97L（P=0．000）；Caveolin-1与HBx表达显著负相关（P=0．044）。结论Caveolin-1表达与HCC侵袭转移密切相关，其表达下凋，预示HCC的预后不良；有可能存在HBx蛋白下凋Caveolin-1表达的机制。

Objective To study the correlation between the expression of caveolin-1 in hepatitis B- related hepatocellular carcinoma （HCC） and the relationship thereof with the invasive and metastatic ability of HCC. Methods Real-time PCR and immunohistochemistry were used to detect the expression of caveoLin-1 in 123 specimens of HCC tissues obtained during operation. Human HCC cells of the lines MHCC-97L and MHCC-97H were cultured, and Western-blotting was used to detect the caveolin-1 expression in these cells. Results The expression level of caveolin-I in the HCC tissues was significantly lower than that of the adjacent noncancerous tissues （P = 0.026）. The caveolin-1 mRNA expression in the HBV-related HCC cells was negatively correlated with the tumor size, expression of AFP, major venous invasion, being single or multiple, pTNM staging, and HBx expression level, factors closed to the prognosis of HCC. The caveolin-1 protein expression of the MHCC-97H cells was significantly Lower than that of the MHCC-97L cells. Conclusion Caveolin-1 expression is associated with the invasive and metastatic ability of HCC, low expression of caveolin-1 shows poor prognosis of HCC, and caveolin-1 is probably downregulated by HBx protein.