期刊文献+
任意字段
题名或关键词
题名
关键词
文摘
作者
第一作者
机构
刊名
分类号
参考文献
作者简介
基金资助
栏目信息

白藜芦醇合酶基因的克隆与植物表达载体的构建 被引量：3

下载PDF
导出
摘要 目的为获得含有白藜芦醇(Res)的转基因植物,进行了白藜芦醇合酶(RS)基因的克隆、植物表达载体构建的研究。方法以葡萄为材料,从其叶片中提取基因组DNA,并以此DNA为模板,利用PCR法扩增得到RS基因,将此基因连接到克隆载体PGEM-TVector,得到重组载体pT-RS;经酶切及测序鉴定后,将RS基因克隆到植物表达载体pBI121,得到重组载体pBI-RS,用PCR及酶切方法进行鉴定。结果重组质粒pT-RS的测序结果表明,RS基因的片段大小为1.522kb。将此片段正向插入植物表达载体pBI121的CaMV35s启动子和NOS终止子之间,对重组子进行PCR及酶切鉴定,均得到预期大小的片断,证明RSDNA与质粒pBI121已成功连接,植物表达载体pBI-RS构建正确。结论成功扩增得到RS基因及成功构植物表达载体pBI-RS,为RS转基因生菜的研究奠定了基础。
作者 朱春燕 雷建军 周浩 吴敏 陈清 陈燕珍 杨素华 俞守义
机构地区 广州医学院公共卫生与全科医学学院 华南农业大学园艺学院 南方医科大学公共卫生与热带医学学院流行病学系
出处 《南方医科大学学报》 CAS CSCD 北大核心 2008年第11期2051-2053,共3页 Journal of Southern Medical University
关键词 白藜芦醇合酶基因 PCR 植物表达载体
分类号 Q943.2 [生物学—植物学]
  • 相关文献

参考文献13

  • 1Bertelli A, Giovannini L, Stradi R, et al. Kinetics of trans- and cis-resveratrol (3, 4', 5-trihydroxystilbene) after red wine oral administration in rats[J].Int Clin Pharmacol Res, 1996, 16(4-5): 77-81.
  • 2Hain R, Reif HJ, Krause E, et al. Disease resistance results from foreign phytoalexin expression in a novel plant [J].Nature, 1993, 361: 153-6.
  • 3Ates O, Cayli S, Altinoz E, et al. Neuroprotection by resveratrol against traumatic brain injury in rats [J].Mol Cell Biochem, 2007, 294: 137-44.
  • 4Holme AL, Pervaiz S. Resveratrol in cell fate decisions [J]. J Bioenerg Biomembr, 2007, 39: 59-63.
  • 5Yilmaz O, Keser S, Tuzcu M, et al. Resveratrol (trans-3,4', 5-trihydroxystilbene) decreases lipid peroxidation level and protects antioxidant capacity in sera and erythrocytes of old female Wistar rats induced by the kidney carcinogen potassium bromate [J]. Environ Toxicol Pharmacol, 2007, 24: 79-85.
  • 6Mokni M, Elkahoui S, Limam F, et al. Effect ofresveratrol on antioxidant enzyme activities in the brain of healthy rat [J].Neurochem Res, 2007, 32:981-7.
  • 7Elmali N, Esenkaya I, Hanna A, et al. Effect of resveratrol in experimental osteoarthritis in rabbits [J]. Inflamm Res, 2005, 54: 158-62.
  • 8Cheng TH, Liu JC, Lin H, et al. Inhibitory effect of resveratrol on angiotensin II-induced cardiomyocyte hypertrophy[J]. Naunyn Schmiedebergs Arch Pharmacol, 2004, 369: 239-44.
  • 9Miura D, Miura Y, Yagasaki K. Resveratrol inhibits hepatoma cell invasion by suppressing gene expression of hepatocyte growth factor via its reactive oxygen species-scavenging property[J]. Clin Experiment Metast, 2004, 21 : 445-51.
  • 10Jang M, Cai L, Udeani GO, et al. Cancer chemopreventive activity of resveratrol, a natural product derived from grapes [J]. Science, 1997, 275: 218-20.

二级参考文献42

  • 1何水林.The phytoalexin Metabolism and Defence Reaction in Plant[M].Guangzhou:Guangdon Science and Technology Publishing Company,2002.1-25.
  • 2田文中 丁力 曹守云 et al.Rice transformation with a phytoalexin gene and bioassay of the transgenic plants[J].植物学报,1998,40:803-808.
  • 3Aguamah G E, Langcake P, Leworthy D P, et al.Two novel stilbene phytoalexins from Atachis hypogaea. Phytochemisay, 1981.20:1381-1383.
  • 4Chen G, Luo H, Ye J, et al. Identification and determination of oligomeric stilbenes in the roots of Caragana species by capillary electrophoresis. Planta Med, 2001,67:665-668.
  • 5Lin J K, Tsai S H. Chemoprevention of cancer and cardiovascular disease by resveratrol. Proc Natl Sci Counc Repub China B, 1999, 23(3):99-106.
  • 6Wanjala C C, Majinda R R. A new stilbene glycoside from Elephnntorrhiza goetzei. Fitoterapia, 2001,72:649-655.
  • 7Subba Rao P V, Strange R N. Chemistry, biology and role of groundnut phytoalexins in resistance to fungal attack. Daniel M. Handbook of Phytoalexin Metabolism and Action, NewYork:Dekkar. 1995.199-227.
  • 8Chan M M. Antimicrobial effect of resveratrol on dermatophytes and bacterial pathogens of the skin. Biochem Pharmacol, 2002,63(2):99-104.
  • 9Privat C, Telo J P,V, et al. Antioxidant properties of trans-epsilon-viniferin as compared to stilbene derivatives in aqueous and nonaqueous media. J Agric Food Chem, 2002,50:1213-1217.
  • 10Zhou C X, Kong L D, Ye W C, et al. Inhibition of xanthine and monoamine oxidases by stilbenoids from Veratrum taliense. Planta Med, 2001,67(2):158-161.

共引文献97

同被引文献48

引证文献3

二级引证文献4

南方医科大学学报
2008年 第11期

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部