木黄酮对培养肝星状细胞增殖及脂质过氧化的影响

Effect of genistein on hepatic stellate cell proliferation and lipid peroxidation in vitro

目的以培养的大鼠肝星状细胞(HSC)为模型,测定植物雌激素木黄酮对HSC增殖及培养液中脂质过氧化产物的影响,以明确木黄酮抑制肝纤维化形成的作用。方法采用培养的SD大鼠肝星状细胞(HSC-T6)为模型,培养后加入H2O2诱导氧化应激,分为3组,用不同浓度的木黄酮温育48h,收集细胞培养基的上清液,每个浓度设6个重复组,用MTT法检测HSC增殖;收集细胞培养基上清液,采用试剂盒方法测定脂质过氧化产物超氧化物歧化酶(SOD)、丙二醛(MDA)、还原型谷胱苷肽(GSH)和谷胱甘肽过氧化物酶(GSH-PX)。结果木黄酮各剂量组不同程度降低HSC的增殖,呈剂量-效应关系。给予木黄酮后,MDA和GSH水平明显降低,SOD和GSH-PX活力明显升高,也呈剂量-效应关系。结论植物雌激素木黄酮具有抑制肝纤维化形成的作用,其作用机制可能与抑制HSC的增殖及抗HSC氧化应激、抗脂质过氧化作用有关。

Objective To investigate the effect of genistein on the proliferation and lipid peroxidation of hepatic stellate ceils （HSC） in vitro and its the protective effect against hepatic fibrosis. Methods Rat hepatic stellate cells （HSC-T6 cells） were divided into 3 groups and incubated in the presence of 0.1 mol/L hydrogen dioxide followed by washing with PBS for 3 times. Genistein at different concentrations was added into the cell culture meclia, and after 48 h of incubation, the cell proliferation was assessed with MTT assay and the levels of superoxide dismutase （SOD）, malonaldehyde （MDA）, glutathione （GSH） and glumthione peroxidase （GSH-PX） in the supematant of the cell culture were measured. Results Genistein at different concentrations inhibited the cell proliferation, showing a dose-effect relationship. Genistein significantly decreased the production of intracellular MDA and GSH and increased SOD and GSH PX activity. Conclusion Genistein can prevent the formation of hepatic fibrosis probably by decreasing HSC proliferation and lipid peroxidation.