表皮生长因子受体和Ki-67抗原在脑室管膜瘤患者肿瘤组织中的表达

Expression of epidermal growth factor receptor and Ki-67 antigen in brain ependymoma and the correlation between them

目的探讨表皮生长因子受体（EGFR）和Ki-67抗原在脑室管膜瘤中的表达及二者的相关性。方法利用免疫组织化学方法检测40例不同级别脑室管膜瘤患者肿瘤组织中EGFR和Ki-67抗原的表达情况以及与肿瘤增值活性的相关性。结果EGFR表达阳性率在Ⅰ～Ⅱ级和Ⅲ-Ⅳ级中分别为25％和75％，差异有统计学意义（P〈0．05）；Ki-67抗原标记指数（u）在脑室管膜瘤I～Ⅱ级为（2．3±1．4）％，Ⅲ-Ⅳ级为（9．5±5．6）％，二者比较P〈0．05。结论EGFR表达阳性率随肿瘤恶性程度增加而升高；EGFR表达阳性率复发组高于原发组；Ki-67抗原和EGFR在不同级别脑室管膜瘤中表达具有正性相关性。

Objective To study the expression of epidermal growth factor receptor （EGFR） and Ki- 67 antigen in human brain ependymoma and the correlation between them. Methods Immunohistochemistry was used to detect the expression of EGFR and Ki-67 antigen in 40 specimens of brain ependymoma, 8 specimens of normal brain tissues, and 8 specimens of glioblastoma, all obtained during operation. Results The EGFR positive rate of the Ⅲ-Ⅳ grade ependymoma tissues was 75%, significantly higher than that of the grade Ⅰ-Ⅱ grade ependymoma tissues （25% , P 〈 0. 05 ）. The specimens of glioblastoma were all EGFR positive. The EGFR positive rate of the recurrent ependymoma tissues （5/6） was significantly higher than that of the primary ependymoma tissues （9/34, P 〈 0. 05）. The positive rate of Ki-67 L1 in the Ⅲ-Ⅳ grade ependymoma tissues was （9. 5±5. 6）%, significantly higher than that in the grade Ⅰ-Ⅱ grade ependymoma tissues [ （ 2.2 ±1.4）% ,P 〈0. 05） ]. The positive rate of Ki-67 L1 in the glioblastoma tissues was （9. 8 ±6. 3 ）%. The positivity of EGFR was positively correlated with the grade level of ependymoma and Ki-67 L1 positivity （ both P 〈 0. 01 ）. Conclusion EGFR expression positive rate increases with tumor grading. The positive rate of recurrent ependymoma is higher than that of the primary ependymoma. The expression of Ki-67 antigen is positively correlated with the expression of EGFR in difference grades.