尖锐湿疣凹空细胞的观察

The Observation of koilocytes in Condylomate Acuminata.

本文对９７例尖锐湿疣（ＣＡ）进行聚合酶链反应检测人乳头瘤病毒（ＨＰＶ）ＤＮＡ及光镜下观察凹空细胞的特征。结果显示，９５例有凹空细胞，占９７．９４％。凹空细胞多分布在棘层的中、上层。根据核的改变将凹空细胞分为九型，以镰刀形核、毛虫样核和核大浓染型出现例数最多，分别为７５／９５、７４／９５和６１／９５，此三型对ＣＡ最有诊断价值。ＨＰＶ６、１１阳性９３例（９５．８８％），ＨＰＶ１６、１８、３１、３３、５２ｂ、５８阳性２例（２．０６％），ＨＰＶ６、１１、１６、１８、３１、３３、５２ｂ、５８阴性２例（２．０６％），但有典型的凹空细胞。２例ＣＡ无凹空细胞而ＨＰＶ６、１１为阳性。

In this paper we detected Human Papillomaviruses (HPV) DNA sequences in skin biopsies obtained from skin lesions in 97 patients with condyloma acuminata using polymerase chain reaction.The skin biopsy specimens fron 97 patients with condyloma acuminata were observed by microscopy.The results showed that koilocytes were present in 95 cases (97.94%) and most koilocytes were located upper and middle of squamous layer.Based on the characteristic of koilocyte nuclei,we divided into 9 distinct koilocytosis types.The sickle-shaped,caterpillarlike-shaped,and large with dense chromosome-shaped nuclei were present in most cases of condylome acuminata and were important for diagnosing condyloma acuminata.93 cases were positive to HPV6、11(95.88%),2 cases were positive to HPV16、18、31、33、526、58(2.06%),2 cases with kolocytes were negative to HPV6、11、16、18、31、33、526、58(2.06%),2 cases with no koilocytes were positive to HPV6、11.