川崎病血清诱导单核细胞表达PDGF及对内皮细胞凋亡的影响

Effects of Serum of Kawasaki Disease on PDGF and on Endothelial Cell Apottosis

应用免疫细胞化学方法和流式细胞术，分别观察川崎病血清诱导人单核细胞表达血小板源生长因子（PDGF）B链蛋白（PDGF－β）的影响和该单核细胞条件培养基对内皮细胞凋亡的影响。结果发现，川崎病血清能强烈诱导单核细胞表达PDGF－β，可显著促进内皮细胞凋亡。提示，单核细胞表达PDGF增多及单核细胞介导的内皮细胞调亡，可能参与了川崎病冠脉并发症的形成。

The effects of serum of kawasaki disease on 5 chain protein of platelet de rived growth factor (PDGF-5)were studied by immunocytochemical method. Mean while,the effects on the endothelial cellu1ar apottosis were investigatedby flow cy tometry technique. It was found that the serum of kawasaki disease induce the ex pression of PDGF-5 chain protein strongly . Likewise,the endothelial cellular apotto sis was increased conspicuously in the experimental group compared with the control group(P<0.01). The results suggest that PDGF increased and endothelial cellular apottosis play a important role in coronary artery complication of kawasaki disease.