水稻新广亲和基因的初步分子定位

Mapping a New Gene for Wide Compatibility of Rice in Relation to RFLP Markers

对02428与IR24、Pecos杂交分离世代的小穗育性的遗传分析表明，02428／IR24F1表现为半不育，不育性受单基因控制，且由非S-5的新位点引起，美国品种Pecos具有复等位中性基因。随机选取分布于12条染色体上的87个探针，结合7种限制性内切酶，对亲本进行了限制性片段长度多态性（RFLP）检测分析，发现其中35个探针能够揭示出亲本间多态性。利用35个表现多态性的探针对Pecos／IR24／／02428三交组合后代F155个植株小穗育性的分离进行分析，第11染色体上的RFLP标记RG98和G24与Pecos中的未知籼粳杂交亲和性基因有显著连锁关系。根据小穗育性和RFLP标记的共分离资料，初步绘制了第11染色体上该广亲和基因S－p（t）座位的RFLP连锁图。

Genetic analysis was conducted on the spikelet fertility in segregation population of IR24, Pecos,02428 crosses. Thirty-five of 87 RFLP probes located on 12 chromosomes combined with 7 restriction enzymes, candetect polymorphisms among the cultivars. These 35 probes were used to further analyze the segregation of spikeletfertility of 55 plants in segregating population of triple cross (Pecos /IR 2 4