益气温阳活血方对慢性心衰大鼠心室重构NF-κB表达的影响

Effects of YiqiWenyangHuoxue decoction on NF-κB in the myocardial remodeling of rat with chronic heart failure

目的:探讨中药复方益气温阳活血方对慢性心衰大鼠心室重构核因子-κB(NF-κB)的影响。方法:体内实验采用大剂量异丙肾上腺素诱导慢性心衰模型,外试验血管紧张素Ⅱ作用48h诱导心肌肥厚、制作肥厚心肌模型。观察模型大鼠心脏指数变化,应用酶联免疫吸附(ELISA)法检测肿瘤坏死因子-α(TNF-α)、白介素-18(IL-18)含量,以逆转录聚合酶链反应(RT-PCR)法以及蛋白免疫印记法(Western-blot法)检测NF-kB mRNA及蛋白质表达水平在益气温阳活血方及拆方治疗前后的变化。结果:模型组TNF-α、IL-18含量及NF-κB mRNA与蛋白质的表达水平明显升高;益气温阳活血方能够明显减少TNF-α、IL-18含量及抑制NF-κB mRNA与蛋白质的表达水平的升高。结论:NF-κB在慢性心衰心室重构机制中起重要作用;益气温阳活血方可能通过抑制NF-κB逆转心室重构,改善心脏功能。

Objective： To investigate the effects of YiqiWenyangHuoxue decoction on NF-κB in the myocardial remodeling of rat with chronic heart failure （CHF）. Methods： Heart failure medel was established with flushing dose isoproterenol in rats and cardiac hypertrophy with angiotensin Ⅱ （Ang Ⅱ ） stimulation 48h in myocardial cell.The model rats and cardiocytes with CHF were treated with YiqiWenyangHuoxue decoction,then cardiac index in model rats was measured;TNF-α ,IL-18 were determined by ELISA; NF-κB mRNA evaluated with RT-PCR,and protein by Western-blot. Results： Compared with the control group, TNF-α ,IL-18 concentration were significantly higher, as well as NF-κB mRNA and protein levels not only in myocardial tissues but also in cardiocytes of CHF groups.But those can be attenuated by YiqiWenyangHuoxue decoction.Conclusions：NF-κB plays important roles in the myocardial remodeling of CHF;and YiqiWenyangHuoxue decoction may reverse the myocardial remodeling through blocking NF-κB.