微管相关蛋白_(30)和ubiquitin在老年性痴呆病神经元纤维缠结中的表达

Microtubuleassociated protein_(30) (MAP_(30)) and ubiquitin detectedin neurofibrillary tangles of Alzheimer's disease brains

采用尸检脑组织标本，经免疫组织化学染色观察微管相关蛋白３０（ＭＡＰ３０）和ｕｂｉｑｕｉｔｉｎ免疫反应产物在老年性痴呆病（ＡＤ）患者海马内神经元的分布及变化，探讨微管相关蛋白与ＡＤ发病机制之间的关系。ＭＡＰ３０和ｕｂｉｑｕｉｔｉｎ免疫染色显示海马内神经元的神经纤维缠结（ＮＦＴ）。ＭＡＰ３０与ｕｂｉｑｕｉｔｉｎ免疫反应阳性神经元密度差异无显著性；二者在ＣＡ１和ＣＡ２区阳性神经元数密度明显高于ＣＡ３及ＣＡ４区。结果表明ＭＡＰ３０和ｕｂｉｑｕｉｔｉｎ参与ＮＦＴ的形成。

Using immunohistochemical technique, the distribution of immunoreactive products of antiubiquitin and MAP_(30) in the neurons of hippocampus of Alzheimer's disease (AD) brain were studied. Nurofibrillary tangles (NFT), dystrophic neurites in plaques and neuropil threads were labeled by the antibody of ubiquitin. NFT and pretangle neurons (PTN) were stained by the antibody of MAP_(30), but not detectable signal in axons or distal dendrites. The neurons immunostained with antibodies to ubiquitin and MAP_(30) were found in all the areas of CA1,CA2,CA3 and CA4 of hippocampus of AD. These neurons labeled by 2 antibodies used in this study were distributed more in the CA1 and CA2 than those in CA3 and CA4. The results indicat that ubiquitin is seen associated when NFT is already formed, and MAP_(30) may be a novel antigenic component of NFT in Alzheimer's disease.