SLE患者白介素1受体拮抗基因多态性分析

Study of interleukin-1 receptor antagonist gene polymorphism in systemic lupus erythematosus

应用聚合酶链反应技术对60例SLE患者和85例正常对照组的白介素1受体拮抗剂（IL－1ra）基因数目可变的串联重复多态性进行了分析。发现SLE患者中IL1RN2等位基因发生频率明显高于正常对照组（P＜0．05）。如将患者分为符合ARA诊断标准4～5项和6项或6项以上两组时发现，前者IL1RN2等位基因频率与正常对照组相比无显著差异（P＞0．05），而后者显著高于正常对照组（P＜0．01）。表明IL1RN2可能不直接影响SLE易感性，但有IL1RN2者患SLE后可能病情更严重。

Using polymerase chain reaction (PCR) to analyze the polymorphism of variable number of tandem repeat(VNTR) of interleukin-1 receptor antagonist (IL -1ra) gene among patients with SLE and normal controls in the present study. The results indicated that the frequency of IL1RN 2 allele was much higher in SLE than that of controls(p< 0. 05). If the patients were classified into two groups, the one meeting 4~5 and the other at least 6 of the American College of Rheumatology(ARA) revised criteria for SLE, we found that there was no significant difference in the frequency of IL1RN 2 allele between the former group and controls (P > 0. 05 ), but the frequency of IL1RN 2 was significantly increased in the latter group compared with controls(p < 0. 01 ). Itsuggests that IL1 RN 2 allele might not directly affect the susceptibility to SLE, but the condition might be more severe for those patients with IL1RN 2.