中药复方促使T淋巴细胞增殖的实验研究

Study on compound traditional Chinese medicine promote to T-lymphocyte proliferation experiment

目的研究中药复方促使T淋巴细胞增殖。方法从SD大鼠血液里分离淋巴细胞,进行体外培养。设正常细胞对照组、阳性药物对照组、转移因子组、复方中药高剂量组、中剂量组、低剂量组。培养1d后把药加入培养液中,继续培养2d,加Am-Blue细胞增殖与活性检测试剂,避光培养4h后,通过酶标仪检测,分析淋巴细胞的增值情况。结果中药复方高、中、低剂量组OD值均明显增高,与正常细胞组比较P<0.01;与阳性药物对照组比较,P<0.01;高剂量组与中、低剂量组比较P<0.01;转移因子组与正常细胞组比较P<0.01。结论中药复方能促使T淋巴细胞增殖,且与剂量有关。

Objective Study of compound traditional Chinese medicine can promote to T-lymphocyte proliferation. Methods Separate T-lymphocyte from blood of SD rat,culture in vitro. Install normal cell control group, active-drug control group, transfer factor group, compound traditional Chinese medicine high dose group, middle dose group, low dose group. Give the medicine to culture medium after culture one day. Continue to culture 2 days and take Am-Blue into 96 orifice plate, culture 4 hours in dark. Use enzyme-labelling measuring instrument to measure after three days. Analyse proliferation of T-lymphocyte. Results OD of compound traditional Chinese medicine high dose group, middle dose group, low dose group can improve obviously. They compare to blank control group P 〈0. 01; they compare to active-drug control group P 〈0. 01 ; compound traditional Chinese medicine high dose group compare to middle dose group,low dose group P 〈 0. 01. transfer factor group compare to blank control group P 〈0. 01. Conclusion Compound traditional Chinese medicine can promote to T-lymphocyte proliferation, and relation to dosage.