早期胃腺癌与胃粘膜上皮细胞内β-葡糖醛酸酶免疫电镜比较研究

Study on the comparison of the immuno electron microscope of βglucuronidase between early period gastric adenocarcinoma cell and gastric mucosa epithelial cell

目的：早期胃癌与胃粘膜上皮细胞内β－葡糖醛酸酶（β－Ｇ）免疫电镜定位、定量，并进行比较，探索β－Ｇ在两者之间的差异。方法：采用低温包埋、紫外线照射、胶体金标记、免疫电镜技术。结果：β－Ｇ定位于早期胃癌细胞与胃粘膜上皮细胞中的溶酶体与内质网内，早期胃癌细胞中β－Ｇ量多于胃粘膜上皮细胞。结论：结果提示β－Ｇ的增多可能与癌细胞旺盛的机能活动和异常的形态结构有关。

Purpose βglucuronidase (βG) was localized and rationed and compared in early phase gastric carcinoma cell and gastric mucosa epithelial cell. The differentiation of βG was explored between above two. Methods Technique of low temperature embedding, ultraviolet rays shining, colloid gold labelling and immuno electron microscope were used. Results βG was localized in lysosome and endoplasmic reticulum, ration of βG was more in early phase gastric carcinoma cell than gastric mucosa epithelial cell. Conclusions This result suggests that the increase of βG might be concerned with the vigorous function and the abnormal morphology structure of the carcinoma cell.